Plasmid-dependent phage represents a unique phage type that recognizes plasmid-encoded sex apparatus (i.e. pilus) as the receptor for gain of entrance into the bacterial cell. Such phage type has also been previously reported as potential novel phage candidates that limit the spread of antibiotic...
What is the definition of plasmid? What are the differences between episomal and integrative plasmids? And what are the benefits and downside of using them for heterologous protein expression in S. cerevisiae? What is the role of bacterial proteins in DNA cleavage?
Introducing the bacterial `chromid': not a chromosome, not a plasmid. Trends Microbiol. 18, 141-148.Harrison PW, Lower RPJ, Kim NKD, Young JPW. 2010. Introducing the bacterial "chromid": not a chromosome, not a plasmid. Trends Microbiol. 18:141-148....
What is the general name for plasmids that have the potential to integrate into the bacterial chromosome? What is a plasmid and why are they important for genetic engineering? a. What is a plasmid? b. Why are they important for genetic engineering?
These findings probably reflect what has been observed in other bacterial species: prophage acquisition and loss is quite dynamic, and the gene pool of phages that infect the B. cereus group is larger and more diverse than that of the rest of the chromosome [1, 13–16]. Prophages, like ...
FM2.5 displays an aberrant colony morphology that is easily distinguished from wild-type C. difficile. Recombination between the plasmid-borne slpA gene and the mutated copy on the chromosome was detected by reversion to normal colony morphology. Plasmid curing was confirmed by loss of thiamphenicol ...
By nature, humans are inquisitive animals: this is the foundation of scientific investigation. However, we sometimes have difficulty in comprehending, and therefore interpreting, the data; this is especially true when working with systems much different
So, they simply designed a plasmid. This plasmid is a piece of DNA. And it contains pieces that can be transcribed to every component of these(crRNAs, tracr RNA, Cas9, DNA Repair Template) So we can have several repeats of CRISPR RNAs(2 repeats as below) ...
The production of β-galactosidase in the strain with the multi-copy plasmid decreased rapidly when growth was performed under induced conditions and without antibiotic selection. In contrast, the strain with the five copies of ganA in the chromosome produced β-galactosidase for at least 40 ...
Bacterial strains and plasmids construction The C. crescentusmCh strain was obtained by conjugation between CB15N and E. coli S17-1 λpir carrying the pXbiofab-mcherry mobilizable plasmid, which allows the constitutive expression of mcherry from the synthetic promoter Pbiofab and includes a 2-kb...