Here we describe an optimized protocol for transient transfection of miRNAs and siRNAs in combination with plasmid DNA. In this experimental set-up, plasmid DNA is a luciferase reporter that allows quantitative measurements of the biological effect of the overexpressed microRNA on the transcription ...
Optimization of a lipitoid-based plasmid DNA transfection protocol for bovine trophectoderm CT-1 cells Embryo-derived cell lines are important in vitro models for investigating the molecular mechanisms directing embryonic tissue lineage segregation and maint... AT Schiffmacher,CL Keefer - In Vitro Cell...
Transfection protocol ComponentCat. No. Invitrogen Lipofectamine 3000 Transfection ReagentL3000008 Gibco Opti-MEM I Reduced Serum Medium31985062 Thermo Scientific Nunc 24-Well Cell Culture–Treated Multidish142475 On the day of transfection, which should be 1 day following cell plating, perform ...
Purity gradeMolecularTransfectionAdvanced transfection Endotoxin level>10 EU/µg0.1–1 EU/µg<0.1 EU/µg Learn more:Plasmid DNA purity grades Robust purification of plasmid DNA, in the amount and purity required for the downstream application of interest, is the key step of any protein expre...
One of the major constraints to performing large-scale transfections of cultured mammalian cells for the transient expression of recombinant proteins is the production of large quantities of purified plasmid DNA. In this report partially purified plasmid DNA was prepared by a method that combines alkal...
Transfection efficiency of plasmid DNA/importin 尾 conjugates consisting of biotinylated poly-ethylenimine and recombinant streptavidin-fused importin 尾.Kawazu, TAzuma, HNagasaki, T
The long-awaited breakthrough was accomplished by US scientists Stanley Cohen, Annie Chang, and Leslie Hsu, who in 1972 demonstrated that a previously established protocol for calcium chloride-mediated transfection of E. coli, the work horse of molecular genetics, with the bacteriophage lambda DNA ...
Efficient DNA removal is integrated into a streamlined, universal protocol and is accomplished without ... P Brisco,M Mandrekar,R Bitner,... 被引量: 1发表: 2006年 加载更多 研究点推荐 Transfection-Quality Plasmid DNA PureYield™ 0关于我们 百度学术集成海量学术资源,融合人工智能、深度学习、...
Kunert R, Vorauer-Uhl K: Strategies for efficient transfection of CHO-cells with plasmid DNA. Methods in molecular biology 2012; 801:213-26Kunert R, Vorauer-Uhl K (2012) Strategies for efficient transfection of CHO-cells with plasmid DNA. Methods Mol Biol 801:213–226. : 10.1007/978-1-...
RFect Plasmid DNA Transfection Reagent是我公司研发团队经过上百次实验、在DeofectEU Transfection Reagent的基础上进一步优化研发成功的专门用于质粒DNA细胞转染的转染试剂。RFect Plasmid 具有非常优异的转染性能,可高效转染多种贴壁细胞,转染效率在贴壁细胞中可高达90%以上(Hela细胞、EGFP质粒)。与目前市场上常见的脂质体...