質體DNA製備規格 Mini製備Mid製備Max製備Mega製備Giga製備 隔夜細菌培養量1–5 mL25–100 mL100–500 mL500 mL–2.5 L2.5–5 L 預估產量Up to 40 µgUp to 400 µgup to 1.5 mgUp to 5 mgUp to 15 mg Purity grade and endotoxin level for plasmid DNA isolation ...
The Nucleic Acid Protocols Handbook: Extraction and Purification of Plasmid DNA. Totowa: Humana Press Inc; 2000.Winstanley, C. and Rapley, R., (2000). "The Nucleic Acid Protocols Handbook: Extraction and Purification of Plasmid DNA", Humana Press Inc., Totowa, NJ,....
Before starting: If you are using the PureLink HiPure Plasmid FP Maxiprep kit and you are precipitating the DNA using the PureLink HiPure Precipitator Module, refer to the precipitator protocol. To precipitate DNA using centrifugation, follow ...
This time- and cost-effective protocol does not require centrifugation or precipitation steps and has the potential for automated DNA extraction, especially within miniaturized lab chip applications. 展开 关键词: Template-blocking PCR Cassette ligation-mediated PCR Genome walking ...
AquaStool mouse fecal DNA extraction protocol 1. Collect the fecal sample Transfer a mouse to a cage floored with a clean paper towel. Scoop up a fecal pellet with a microfuge tube labeled with the animal ID (fecal pellets may be stored at RT for months after being air-dried by incubating...
申请(专利)号: US2007/016497 申请日期: 07/21/2007 公开/公告号: WO/2008/121121 申请(专利权)人: 发明人: X Jia 国省代号: WO 被引量: 8 摘要: The invention provides apparatus, reagents, and methods for rapidly isolating plasmid DNA from a bacterial alkaline lysate.收藏...
Optimization of a DNA Extraction Protocol for the Beckman Coulter Biomek® NXP Laboratory Automation Workstation DNA analysis plays an integral role in forensic science investigations. This results in a high volume of samples requiring DNA testing. Due to the growing ... ASY Liu,OO Chief 被引量...
restriction analysis and sequence data. Third, the basis for nearly all modern methods of plasmidDNA extractionwas established by HC Birnboim and J Doly[5]withalkaline lysisofbacterial cellsfollowed by neutralization with potassium acetate precipitating the chromosomal DNA in a complex with other cell...
The QuickClean 96-Well Plasmid Miniprep Kit is designed to purify up to 20 μg/well high-purity plasmid using 96-well plates in less than 60 minutes by centrifugation. Cells are lysed, filtered and the plasmid DNA is bound to a silica membrane plate. Then the plasmid DNA is washed and ...
Protocol overview Resources PublicationsDESCRIPTION The FavorPrep™ Plasmid Extraction Mini Kit provides a rapid, phenol-free method for the extraction of high-purity plasmid DNA from bacterial culture such as E. coli. Silica membrane based DNA column is utilized in the purification process, and the...