質體DNA製備規格 Mini製備Mid製備Max製備Mega製備Giga製備 隔夜細菌培養量1–5 mL25–100 mL100–500 mL500 mL–2.5 L2.5–5 L 預估產量Up to 40 µgUp to 400 µgup to 1.5 mgUp to 5 mgUp to 15 mg Purity grade and endotoxin level for plasmid DNA isolation ...
Plasmid DNA isolation is indivisible step in the development of diagnostic assays based on recombinant proteins and other molecular biology experiments. There are hundreds of protocols published for isolation and purification of plasmid DNA and still the
The Nucleic Acid Protocols Handbook: Extraction and Purification of Plasmid DNA. Totowa: Humana Press Inc; 2000.Winstanley, C. and Rapley, R., (2000). "The Nucleic Acid Protocols Handbook: Extraction and Purification of Plasmid DNA", Humana Press Inc., Totowa, NJ,....
This time- and cost-effective protocol does not require centrifugation or precipitation steps and has the potential for automated DNA extraction, especially within miniaturized lab chip applications. 展开 关键词: Template-blocking PCR Cassette ligation-mediated PCR Genome walking ...
AquaStool mouse fecal DNA extraction protocol 1. Collect the fecal sample Transfer a mouse to a cage floored with a clean paper towel. Scoop up a fecal pellet with a microfuge tube labeled with the animal ID (fecal pellets may be stored at RT for months after being air-dried by incubating...
Carboxyl-coated magnetic nanoparticles for mRNA isolation and extraction of supercoiled plasmid DNA Carboxyl-coated magnetic nanoparticles (MNPs) were used to demonstrate dual functionality: isolation of messenger RNA (mRNA) from mammalian cells and extra......
Protocol overview Resources PublicationsDESCRIPTION The FavorPrep™ 96-Well Plasmid DNA Extraction Kit is designed for high throughput isolation of plasmid DNA. The technology is based on alkaline lysis followed by adsorption of DNA onto silica membrane in the presence of chaotropic salt. The extracte...
Third, the basis for nearly all modern methods of plasmid DNA extraction was established by HC Birnboim and J Doly [5] with alkaline lysis of bacterial cells followed by neutralization with potassium acetate precipitating the chromosomal DNA in a complex with other cell debri. Being historically ...
We developed a new simple high-throughput plasmid DNA extraction procedure, based on a modified alkaline lysis method, using only one 96-well microtiter gl... I Masayoshi,C Piero,N Sumiharu,... - 《Nucleic Acids Research》 被引量: 61发表: 1997年 On the stability of plasmid DNA vectors ...
With close to 20 years of CRO experience, GenScript truly understands your needs. From transformation to extraction, we have developed a robust protocol with years of process optimization to provide you top-notch quality plasmid preps with fully customizable services. ...