Isolation of Agrobacterium tumefaciens from soil and Optimization of Genomic and Plasmid DNA Extraction. International Journal of Advanced Research, 1(2): 1-4.Anil Kumar, Niharika, G, Bhawsar, Poornima Badnagre, Ujjaval Panse, SR, Gayakwad, and Krishna Khasdeo, (2013a). Isolation of ...
Plasmids are extremely valuable as a source of DNA and for use in biotechnology. Our research goal was to develop plasmids with extremely high copy numbers for lab-scale plasmid preparations. Using the beverage method simplifies the process of obtaining a large quantity of plasmid DNA for various...
Bacterial (sheet 1) and plasmid (sheet 2) microbial abundance matrix. Supplementary Information 14 Gammaretrovirus-like ERV sequences identified in D. rotundus genome. Supplementary Information 15 Statistically significant antiviral genes deeper selection analyses. Sheet 1: M1vsM2 test. Sheet 2: M8av...
fragments of 2–3kb and 7–10kb are ligated into a pUC18 and pTWV228 plasmid vectors respectively. The ligated DNA samples are introduced intoE. coliby electroporation that resulted in two genomic libraries. Sequencing is carried out from both the ends of plasmid DNA using ABI-3700 capillary ...
The primary safety concern for DNA vaccines is their potential to integrate into host cellular DNA. We describe a sensitive and quantitative assay for investigating the tissue distribution and integration of plasmid DNA vaccines. By including gonadal tissues in the analysis, the potential for germline...
Plasmid Purification & Colony Screening DNA Clean Up & Concentration PCR Reagents RT-qPCR Reagents Molecular Cloning and Next-Generation-Sequencing Enzymes RNA Isolation DNA and RNA Detection Yeast Research Tools Molecular Biology Accessories, Buffers & Reagents Bioassays Reagents & Chemicals...
Plasmid DNA was extracted from the bacteria cultures using the QIAprep Mini Kit (Qiagen), and Sanger sequenced to verify the cloned product. The successfully cloned plasmids containing the WT and mutant 5′-UTR sequences of interest were transfected into cell lines using Lipofectamine 3000 (...
(3) Contigs that matched 100% with the Nipponbare or 93-11 nuclear genomic sequences were ignored as they were considered to be nuclear contaminants and, as in the case of 'dead-end' contigs, depth values of these contigs were always under 15. (4) Plasmid-like sequences, corresponding ...
Workflow of the sequencing and analysis of the YKJ genome. It comprises sample preparation, next-generation sequencing (NGS) and data analyses including genome assembly, mapping reads, gene annotation, phylogenetic studies and identification and extraction of the plasmid DNA sequence. As shown in the...
The PureLink™ Genomic DNA Purification Kit is based on the selective binding of DNA to silica-based membrane in the presence of chaotropic salts. The lysate is prepared fromE. colicells, yeast cells, mouse tails, and mammalian cells and tissues. The cells or tissues are digested with Protein...