PROTOCOL Product User Manuals (PDF) AquaGenomic User Manual Protocols for genomic DNA extraction AquaBluer User Manual Protocols for cell viability, cell proliferation, and cytotoxicity assays AquaPlasmid User Manual Protocols for plasmid DNA extraction ...
Plasmid miniPREP Kit Plasmid PREP Kit Protocol - …:质粒提取试剂盒的质粒制备试剂盒的协议—…质粒,提取,kit,prep,试剂盒,Kit,提取试剂盒,质粒提取,Prep,制备质粒 文档格式: .pdf 文档大小: 1.16M 文档页数: 2页 顶/踩数: 0 / 0 收藏人数: 0 评论次数: 0 文档热度: 文档分类: 论文 -- 毕业...
QIAprep Spin Miniprep Kit High-Yield Protocol - English (PDF) EN PDF (115KB) For purification of up to 30 μg plasmid DNA User-Developed Protocols (4) Isolation of plasmid DNA from Agrobacterium using the QIAprep® Spin Miniprep Kit; spin procedure - (...
QIAGEN Plasmid Plus Kits – Plasmid DNA Extraction For the fastest, most convenient purification of up to 10 mg transfection-grade plasmid DNA Product Product Details Resources FAQ Want to try this solution for the first time? Get in touch with our team today a...
analysis and sequence data. Third, the basis for nearly all modern methods of plasmid DNA extraction was established by HC Birnboim and J Doly[5]with alkaline lysis of bacterial cells followed by neutralization with potassium acetate precipitating the chromosomal DNA in a complex with other cell ...
Rapid separation of plasmid DNA was achieved with high DNA Fragment Plasmid DNA RNA Figure 2. Plasmid DNA Separation after Centrifugation. quality (Figure 1). No crystallization resulted, and the two bands of DNA were well separated. PROTOCOL 2: Using the Thermo Scientific S120-AT2 Fixed Angle ...
The QuickClean 96-Well Plasmid Miniprep Kit is designed to purify up to 20 μg/well high-purity plasmid using 96-well plates in less than 60 minutes by centrifugation. Cells are lysed, filtered and the plasmid DNA is bound to a silica membrane plate. Then the plasmid DNA is washed and ...
ATP assay system. The intracellular ATP levels of the wild-type and mutant Shigella strains were measured using an ATP bioluminescence assay kit (Promega, USA) as per the manufacturer's protocol. Strains were grown in LB broth to stationary phase, diluted in dilution buffer, and lysed ...
Isolation of endotoxin-free plasmid DNA using the QIAGEN® Plasmid Midi Kit - (EN) EN PDF(50KB) This protocol is for purification of up to 100 µg endotoxin-free plasmid DNA using QIAGEN-tip 100. Isolation of large-construct DNA using the QIAGEN® Plasmid Maxi Kit - (EN) ...
All plasmid DNA was used to transform ancestral MR-1 cells by electroporation, and all transformants (typically 450–1800) were cultured in LB (Km). The MR-1 host-switching protocol was carried out with four independent replicate lineages (Sh11, Sh13, Sh14 and Sh15, corresponding to ...