The pKa of nimesulide was determined by potentiometric titrations of a series of semisqueous nimesulide solutions of known concentration and 34.47–60 Wt% of methanol in water, with a glass electrode, using a pH meter calibrated by means of standard aqueous buffer solutions. The estimated pKa ...
Plug that value into the equation delta G nought = -RTlnK and you will get 7.0 x 10^-14. Most organic chemistry textbooks conjure up the 15.7 value in an attempt to explain the comparative reactivities of methanol, ethanol, and water, but the logic used is completely incorrect. The conce...
D, dioxane; E, ethanol; M, methanol; W, water. ‡ Values obtained by thermometric titrimetry are nearly identical, those got by spectrophotometry are systematically lower (by about 2–20 %). The strong withdrawing action of the DTT-5-yl moiety explains also the low pKavalue of oxime100ai...
47、HCH 2OH4.4643,3 ' ,3 ” -Trimethyl-6.35-5.9566HOCH 2CH2OH14.7764Unsubstituted triphenyl-CH 3CCH 2OH14.8264methanol-6.63-6.896.6066CH3OH15.5464 4,4;,4;-Trichloro-7.74-8.0166CH 2=CHCH 2OH15.52644 -Nitro-9.15-9.7666H2O15.7464CCl 3CH 2OH11.8*CH 3CH 2OH1664CF3CH2OH11.3*Substituent effects...
methanol. In inclusion, no antibacterial task had been discovered for the 75% and 50% methanol ratios. The sum total phenolic amounts had been 16.26%, 12.73%, and 12.33% for the 100%, 75%, and 50% methanol solvent ratios, respectively. The total amounts of flavonoids had been 23.32%,...
Note 2. The pKaof pure water is 14. The relative acidity of water relative to alcohols can change in other solvents. For example, in 2-propanol, methanol is 3 times more acidic than water. See Ref 1. Note 3. How are pKavalues measured? Seereference six. ...
Cells were plated at a density of 1–2 × 105 cells per well on glass coverslips (Fisher #12-545-80P) in 24 well plates. Cells were treated with either 25 µM Forskolin (Cell Signaling technology #3828) or vehicle (DMSO) for 20 min then fixed on ice in ice cold methanol for...
After the indicated treatments (all treatments were performed for 24 h, while ChQ treatment was 4 h), cells were fixed with ice-cold methanol for 15 min, followed by three rinsing in PBS at room temperature. Fixed cells were then permeabilized with 0.3% Triton-X100 in PBS for 10...
was added at final concentrations as indicated from a stock of either 5 or 1 mM in methanol. Methanol addition had no effect on cell length or Pom1 localization. In Fig. 4a,b cells were first grown in EMM with proper supplements, containing 2% glucose to mid-log phase. They were ...
Methanol levels were maintained at 0.05% by the further addition of methanol solution over the course of 72 h until cells were harvested by centrifugation. To harvest, cells were separated from the media by centrifugation at 10,000 × g for 20 min in an F-10 fixed angle rotator at 4−...