D, dioxane; E, ethanol; M, methanol; W, water. ‡ Values obtained by thermometric titrimetry are nearly identical, those got by spectrophotometry are systematically lower (by about 2–20 %). The strong withdrawing action of the DTT-5-yl moiety explains also the low pKavalue of oxime100ai...
Quenching was performed by adding 4 ml buffer 1 (60% methanol with 10 mM tricine) at −80 °C for 5 min. Cells were then centrifuged and resuspended in 400 μl buffer 2 (75% ethanol with 0.5 mM tricine). Cells were lysed by incubating at 80 °C for 3 min ...
For immunofluorescent staining of TJ and AJ proteins, cell monolayers on Transwell inserts were fixed in cold methanol at −20 °C for 20 min and washed with cold DPBS three times. Then cells were blocked with 5 % BSA in DPBS and subjected to immunolabelling as described above, ...
Ssanghwa-tang (SHT) is a widely used medication for the treatment of fatigue, pain, inflammation, hypothermia, erectile dysfunction, cancer, and osteoporosis in Asia, however, role of SHT on the melanin synthesis has not been checked previously. Thus, the present study was designed to determine...