Tris-HCl缓冲液由Tris(三羟甲基氨基甲烷)和HCl(盐酸)组成。Tris是一种弱碱,其在水溶液中能够部分解离出H⁺离子和带负电荷的Tris⁻离子。HCl则是一种强酸,能够提供H⁺离子。通过调整Tris和HCl的比例,可以制备出具有不同pH值的Tris-HCl缓冲液。Tris的pKa值约为8.1,这使得Tris-HCl缓冲液在pH 7.0到9.0范围内...
解:Tris缓冲溶液中加入0.050 mol/L HCl溶液体积为V mL,则n (Tris)-|||-7.40=pK.+lg-|||-n(Trisg·HCl)-|||-0.05×100-0.05V-|||-7.40=7.85+lg-|||-0.05×100+0.05V解得:V=48.1 mL因此,欲配制pH=7.40的缓冲溶液,需加入0.050 mol·L-1HCl 48.1 mL此缓冲溶液中,0.05×100-0.05×48.1-|||...
注意事项:主要由Tris-HCl、DTT、氯化钠、氯化镁组成。 产品货期:现货 上海依赫生物有限公司回馈新老客户,低价活动进行中,促销产品: 核酸助沉剂 1ml/5ml 核酸纯化柱(小柱) 100套 核酸纯化柱(大柱) 10套 PKA缓冲液(10×) DNA退火缓冲液(5X) 5ml DMEM(H) 500ml DMEM(H)(不含酚红,谷氨酰胺,丙酮酸...
1已知Tris·HCl在37℃时的pKa为7.85,今欲配制pH为7.40的缓冲液,问在含有Tris和Tris·HCl各为0.05 mol·L-1的溶液100ml中,需加入0.05 mol·L-1的HCl溶液多少ml? 2已知Tris·HCl在37℃时的pKa为7.85,今欲配制pH为7.40的缓冲液,问在含有Tris和Tris·HCl各为0.05 mol·L-1的溶液100 mL中,需加入0.05 mol·...
Cyclic AMP-dependent protein kinase activity is assayed in a reaction mixture containing, in a final volume of 0.2 mL, 50 mM Tris-HCl (pH 7.0), 10 mM magnesium acetate, 2 mM EGTA, 1 μM cyclic AMP or absence of cyclic AMP, 3.3 to 20 μM [r-32P] ATP (4×105 c.p.m.), 0.5...
The pellet containing the mitochondria-enriched fraction was resuspended in cold supplemented RIPA Buffer 1x (NaCl 150 mM; Tris-HCl 50 mM; NP-40 1%; sodium deoxycholate 0.5%; SDS 0.1%). The supernatant was enriched in the microsomal fraction. Western blotting The cells were lysed in ...
Tris·HCl在37℃时的pKa为7.85,今欲配制pH为7.40的缓冲液,问在含有Tris和Tris·HCl各为0.05 mol·L-1的溶液100ml中,需参加0.05 mol·L-1的HCl溶液多少ml? 相关知识点: 试题来源: 解析 解:设需参加HCl的体积为x mol根据 ,有 解得:x=47.6(ml)...
HEK293T cell pellets were lysed in NP40 lysis buffer (1% NP40, 20 mM Tris-HCL pH 7.5, 150 mM NaCl, 1 mM EGTA, 5 mM NaF) with complete protease inhibitor cocktail (Roche) and N-ethylmaleimide (NEM, Sigma). For immunoprecipitation (IP), cleared lysates were added to either ...
已知Tris·HCl在37℃时的pKa为7.85,今欲配制pH为7.40的缓冲液,问在含有Tris和Tris·HCl各为0.05 mol·L-1的溶液100ml中,需加入0.05 mol·L-1的HCl溶液多少ml? 相关知识点: 试题来源: 解析 解:设需加入HCl的体积为x mol根据 ,有 解得:x=47.6(ml)...
Protein extracts were incubated with anti-c-Myc magnetic beads, pre-equilibrated in wash buffer (500 mM NaCl, 20 mM Tris-HCl pH 8.0, 2 mM MgCl2, 0.5% nonident P40). Beads were washed three times with wash buffer and bound proteins eluted at 95 °C. For the analysis of Maf1 ...