Tris-HCl pH 8.5 VWR 100219-630 Disposable spinner flasks Corning CLS3578 mTeSR basal medium StemCell Technologies, Inc. 85850 MCDB131 Basal Medium Sigma Aldrich M8537 Fetal Bovine Serum Life Technologies 16000044 Fluo-4 Life Technologies F14217 Oligomycin A Fisher Scientific 49545510MG Disuccinyl Sube...
(20 mM Bis–Tris, pH 6.5, 5% glycerol, and 0.5 mM TCEP) and the bound proteins are eluted with 100-2000 mM NaCl gradient. The pooled peak fractions were diluted to ~250 mM NaCl and loaded onto a Q Sepharose FF column equilibrated with Q buffer (25 mM Tris-HCl, pH ...
Total cell lysates from S2 cells were obtained by incubating harvested cells in ice-cold lysis buffer (300 mM NaCl, 100 mM Tris-HCl pH 8, 2% NP-40, 1% deoxycholate, 0.2% SDS, 10 mM EDTA, ‘Complete mix’ protease inhibitors from Roche), followed by centrifugation and denaturation...
Cells were harvested by centrifugation at 10,000 ×g and resuspended in 20 mM Tris–HCl pH 7.4. The cells were disrupted by a single passage though a French Press at 135 MPa and the resulting protein suspension centrifuged at 150,000 ×g for 60 min. The clear supernatant was applied to ...
Peluso JJ, Romak J, Liu X: Progesterone receptor membrane component-1 (PGRMC1) is the mediator of progesterone’s antiapoptotic action in spontaneously immortalized granulosa cells as revealed by PGRMC1 small interfering ribonucleic acid treatment and functional analysis of PGRMC1 mutations. Endocrino...
Figure 1. Methylphenidate HCl structure Methylphenidate HCl is a white, odorless crystalline powder. Its solutions are acid to litmus. It is freely soluble in water and in methanol, soluble in alcohol, and slightly soluble in chloroform and in acetone. ...
[25]. In each well, the reaction mixture contained: 15 µl of 1 M Tris–HCl buffer (pH 8.0; Sigma-Aldrich), 200 µl of 1 mM pNPA (Sigma-Aldrich) dissolved in acetonitrile and 15 µl of sample in GET solution (preparation: 15 µl of liver sample in 60 µl or 30 µ...
Then, cells submitted or not to cross-linking were resuspended in TNM buffer (100 mM NaCl, 300 mM sucrose, 10 mM Tris–HCl, pH 7.4, 2 mM MgCl2 and 1% thiodiglycol v/v) containing 1 mM PMSF, 30 mM sodium butyrate and phosphatase inhibitors. Triton X-100 was added to a final ...
A 25.0 mL sample of a 0.1900 M solution of aqueous trimethylamine is titrated with a 0.2375 M solution of HCl. Calculate the pH of the solution after 30.0 mL of acid have been added; pKb of (CH3)3N = To make 2 L of 0.6% sugar solution, you will need to use ___ mL of the ...
The cells were lysed with 0.8 mL 0.1% Triton X-100 membrane raft isolation buffer [1 M Tris–HCL, pH7.4, 1 M NaCl, 100 mM EDTA, Triton X-100, DW, protease inhibitor cocktail (500×)] by passing through a 23-gauge needle on a 5-mL syringe 20 times. Next, 500 μL supernatant ...