Protein extracts were incubated with EZ red anti-myc affinity beads (Sigma), pre-equilibrated in IPP50 buffer (10 mM Tris pH 8.0, 50 mM NaCl), for 4 h at 4 °C. Beads were washed three times with IPP50 buffer and proteins eluted using 2x BOLT Sample buffer (Thermo Scientific) at ...
To buffer acidic loads and raise the pH in instances of acidosis, Tris-hydroxymethyl aminomethane (THAM), an amino alcohol, is a clinically relevant substance. Sodium bicarbonate, unlike THAM, causes a rise in plasma sodium levels and produces carbon dioxide (CO2) as a consequence of its ...
GFP-Vps4 and the EQ mutant were a gift from Phil Woodman (University of Manchester)37 and GFP-Rab5 Q79L was a gift from Volker Haucke (FMP, Berlin)62. Lysis and immunoprecipitation and western blotting HEK293T cell pellets were lysed in NP40 lysis buffer (1% NP40, 20 mM Tris-...
Initial purification was performed using IEX-FPLC (UPC-900 Amersham Biosciences) and 50 mL of Source 30S anion exchange resin (GE Healthcare). The column was equilibrated with six column volumes of loading buffer, (50 mM Tris-Base, pH 7.5) before loading the supernatant. After loading, the ...
What is the pH of a 250 M solution of TRIS if the pKa is 8.08? What is the pH of a 1.5 M solution of trifluoroacetic acid (CF3CO2H) with pKa = 0.2? What is the pH of a 1.0 M solution of trifluoracetic acid (CF_3CO_2H) with a pK_...
The system involves oxidation of aqueous tris(2,2′-bipyridyl)ruthenium(ii) ions at a glassy carbon electrode on ... C Lledo-Fernandéz,I Hatay,MJ Ball,... - 《New Journal of Chemistry》 被引量: 10发表: 2009年 Proton transfer during the reaction between fully reduced cytochrome c oxidase...
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†pKavaluesreferto25,CunlessotherwiseindicatedValuesinparenthesesareconsideredtobelessreliableDataarefromAEMartell,RMSmith,andRJMotekaitis,NISTDatabase46(Gaithersburg,MD:NationalInstituteofStandardsandTechnology,2001) ‡TheaccuratewaytocalculateKbfortheconjugatebaseispKb(13995)pKaandKb(10)pKb ...
The protein concentration of these samples was determined by a BCA protein assay kit (Beyotime Corp, China). The proteins were then loaded on a Tris-HCl SDS-PAGE gel (Bio-Rad Laboratories, CA, USA) and separated by electrophoresis at 80 V. Then, the proteins were transferred onto PVDF ...
Table S4. Phosphoproteomics Analysis of NCI-H69 and NCI-H526 SCLC Cells in Response to 8-Br-cAMP, Related to Figure 6 Table S5. Factors Identified in Both AS-PKA (Analog-Sensitive) and Global Phosphoproteomic Datasets, Related to Figure 6Show all extrasVolume...