2. Calmagite 3. Dichlorofluoro Scein 4. Phenol red 5. Eriochrome black T 都找不道啊，好心人...
Values of −0.75 (−2.13 on the Hammett acidity scale) for bromocresol purple, +1.05 for cresol red, and +1.03 for phenol red were estimated. pKa values of cresol red and phenol red were not estimated on the Hammett acidity scale, since this scale is good only for acidic solutions ...
Angiogenesis, the growth of blood vessels from pre-exiting vascular structures, is a critical developmental eventin uteroand in the revascularization of damaged or ischemic tissues in the adult1. In addition, angiogenesis contributes, either adaptively or mal-adaptively, to a myriad of conditions incl...
The growth rate of a yeast cell is controlled by the target of rapamycin kinase complex I (TORC1) and cAMP-dependent protein kinase (PKA) pathways. To determine how TORC1 and PKA cooperate to regulate cell growth, we performed temporal analysis of gene e
19F NMR also revealed a pKa of 8.7 ± 0.05 that we attributed to an active-site residue involved in deprotonating bound substrate, and assigned to His-120 based on studies of protein variants. Thus, in both types of hydroxylases, we confirmed that binding favors the phenolate form of ...
and Cav3.3. The black lines represent the control condition, while the red lines indicate the current obtained after the addition of each peptide. The dotted lines represent the 0 current level. The graphs illustrate the effects obtained in a series of at least three independent experiments (n...
They were then incubated for 6 h in glucose- and phenol red-free DMEM (pH 7.4) supplemented with sodium lactate and pyruvate before NATURE COMMUNICATIONS | 7:13147 | DOI: 10.1038/ncomms13147 | www.nature.com/naturecommunications 13 ARTICLE NATURE COMMUNICATIONS | DOI: 10.1038/ncomms13147 ...
Tyr371 is one of the key residues that contributes to capping the B site cyclic AMP into the β-barrel core of domain B. This residue makes hydrophobic interactions by stacking its side chain phenol ring against the adenine ring of cAMP. Its hydroxyl group also contributes by hydrogen bonding...
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Reverse transcription (RT) of 1.5 μg to 1 mg total RNA was performed after RNA extraction with the phenol/guanidine isothiocyanate method followed by DNAse-I digestion [17]. GCMa expression studies were carried out using the LightCycler-FastStart DNA Master SYBR Green kit and the LightCycler Th...