CONCLUSION:PKC, instead ofPKA, wasinvolvedinthesignaltransductionof therapideffectof B onglycineuptakein C6cells. 结论:上述结果提示蛋白激酶C参与了硫酸皮质酮快速抑制C6细胞摄取甘氨酸的信号转导过程。 www.cjpp.net:8080 5. SothisPKAsensornotonlyindicateswhethertheenzymeisonoroff,butalsolocateswherePKAis be...
Cells seeded on glass coverslips were fixed with 4% paraformaldehyde for 10 min, permeabilized with 0.2% Triton X-100/100 mM glycine for 10 min and blocked for 20 min in 3% bovine serum albumin, before primary antibody incubation for 1 h at room temperature or at 4 °C over...
The pKavalues of thecarboxyl groupand the ammonium group of an amino acid, as well as the pHi, can be determined by titrating the conjugate acid withbase.Figure 14.2shows a typical titration curve for glycine. As base is added, some of the conjugate acid is converted to thezwitterion, and...
The effect of the α-amino group, at pH 2, on the hydrophobicity of the side-chain of the N-terminal residues was obtained by referencing the retention time of the acetylated or non-acetylated peptide to the retention time of a glycine analogue. It was shown that the presence of an α-...
48、on of RCH2OHRCCl-312.24,11.8064,65CF3-12.3764CHF2CH2-12.7464Hydroxamic acidsCHCl 2-12.8964Furo-8.4572CHEC-13.5564Glycine7.4072H2Cl-14.3164Hippuro-8.8072CH 3CCH 2-14.864isoNicotin7.8572HOCH 215.164 p-Methylbenz-8.9072H-15.564Nicotin-8.3072CH 2=CH-15.564Nicotin-methiodide6.4672CH 3-(extrap)(15.9...
store at -20 °c. buffer: 50 mm tris-glycine (ph 7.4), 0.15 m nacl, 40% glycerol, 0.01% sodium azide and 0.05% bsa. stable for 12 months from date of receipt. alternative names prkar2a,mgc3606, pkr2, prkar2,camp-dependent protein kinase type ii-alpha regulatory subunit, ...
The crosslinking was performed in 1% formaldehyde and quenched by adding 10 ml of 2.5 M glycine. Harvested cells were resuspended in FA-SDS lysis buffer (0.1% SDS, 40 mM HEPES-KOH, pH7.5, 1 mM EDTA pH 8.0, 1% Triton X-100, 0.1% Na deoxycholate, 1 mM PMSF, 2 μg/...
(in µM): 1 staurosporine, 1 KN93, 2 H-89, 20 PKI, 10 Bis I, 10 Gö6976, 0.1 or 1 OA, 0.1 Ang II, 1 Iso and 10 forskolin. Cultured cardiomyocytes were fixed with 4% PFA for 10 min. Fixed cells were rinsed with 1xPBS + 100 mM glycine, then 1xPBS. Cells were ...
The superposition of X-ray structure of the C / ADP / AlF 3 / PKS complex ( PDB ID 1L3R ) and the C / ATP / PKI complex ( PDB ID 1ATP ) . Glycine-rich loop and catalytic loop are represented as line ribbon and flat ribbon , respectively. The residues from 1ATP are shown ...
pK_a values are given as +H_3N-CH_2COOH 2.0, +H_3N-CH_2-COO-: 9.0; fill in the blanks for the statement below: Glycine may be used as a biochemical buffer. It buffers well in the ~___ to ~___ range. Indicate all acidic hydrogen atoms in the given molecule....