specific for rabbit IgG labeled with peroxidase is also added to the plate to bind to the rabbit anti-phospho-PKA substrate. After a short incubation and wash, substrate is added and the intensity of the color developed is directly proportional to the amount of PKA in the samples and ...
specific for rabbit IgG labeled with peroxidase is also added to the plate to bind to the rabbit anti-phospho-PKA substrate. After a short incubation and wash, substrate is added and the intensity of the color developed is directly proportional to the amount of PKA in the samples and ...
An antibody specifc or rabbit IgG labeled with peroxidase is then added to the plate to bind to the rabbit anti-phospho- PKA substrate. A ter a short incubation and wash, substrate is added and the intensity o the color developed is directly proportional to the amount o PKA in the ...
The Agatston coronary artery calcium (CAC) score effectively predicts cardiovascular disease risk, though its calculation of plaque area is influenced by density. Lorlatinib supplier Density, though, has been shown to be inversely proportional to the occurrence of events. While separately considering ...
S. pombe transformed with a wild-type YpkA-encoding plasmid displayed a pronounced actin mislocalization phenotype, the severity of which was directly proportional to the level of YpkA expressed in the cell. In cells expressing the kinase-inactive YpkA variant, the degree of actin mislocalization...
Hypoxia is a phenomenon often arising in solid tumours, linked to aggressive malignancy, bad prognosis and resistance to therapy. Hypoxia-inducible factor-1 has been identified as a key mediator of cell and tissue adaptation to hypoxic conditions through transcriptional activation of many genes involved...
The PKAC N-terminal domain is colored gray, the C-terminal domain green, the common PCRD/DCRD binding site red, and the unique PCRD binding site magenta Full size image PCRD and DCRD directly interact with PKAC in a concentration-dependent manner To further corroborate the direct ...
Autophagy is a highly regulated degradative process crucial for maintaining cell homeostasis. This important catabolic mechanism can be nonspecific, but usually occurs with fine spatial selectivity (compartmentalization), engaging only specific subcellul
The product formed is proportional to the time of incubation and to the amount of PDK1 and inactive AKT2 added. PDK1 is added at suboptimal levels so that the assay could sensitively detect inhibitors of AKT2 activation as well as direct inhibitor BX912 of PDK1 or AKT2. To measure PDK1 ...
protein(CREB),cAMP response element modulator (CREM), and activating transcription factor 1 (ATF1). Each of these transcription factors contains a kinase-inducible domain containing a conserved site for phosphorylation by PKA. PRINCIPLE The PKA (Protein Kinase A) Activity kit is designed to ...