This method is suitable for monitoring mitochondrial pH shifts under oxidative stress and hypoxia, and for tracking mitophagy processes. The pKa values of probes A and B (641 and 683, respectively), in proximity to physiological pH, facilitate their effective mitochondrial targeting, low cytotoxicity...
Using budding yeast as a model and single-cell methods to dynamically measure metabolic activity, we observe two peaks in protein synthesis, in the G1 and S/G2/M phase, whereas lipid and polysaccharide synthesis peaks only once, during the S/G2/M phase. Integrating the inferred biosynthetic...
When heat is used, complete inactivation can be proportional to the level of heat and time of exposure, whereas in non-thermal food processing complete inactivation might not always occur in a linear fashion65,66. The extent of microbial inactivation depends on multiple factors related to the ...
For a rough estimate of metabolic water production by the human retina, let us assume that the average fraction of metabolic water produced by the tissue is proportional to the fractional blood flow to that tissue. The total blood flow to all tissue for normal adult human is 5000 ml/min, ...
Stronger intracellular acidification of HEK293 cells was induced by citric acid than by HCl and taste-modifying effect of MCL was proportional to intracellular pH regardless of types of acids. These results suggest that intracellular acidity is required for full activation of the sweet taste receptor...
We can learn much about the likely extent of microbial inactivation that can be achieved by thermal and non-thermal processing from studies conducted on food-borne pathogens. When heat is used, complete inactivation can be proportional to the level of heat and time of exposure, whereas in non-...
However, other GPCRs have been linked to PP2A activation via increased PKA activity (32), increased PKC activity (33), and/or increased intracellular Ca2+ levels (34, 35). In order to corroborate mGlu5-mediated PP2A activation, here we tested whether agonist stimulation upregulated striatal ...
Stronger intracellular acidification of HEK293 cells was induced by citric acid than by HCl and taste-modifying effect of MCL was proportional to intracellular pH regardless of types of acids. These results suggest that intracellular acidity is required for full activation of the sweet...
When heat is used, complete inactivation can be proportional to the level of heat and time of exposure, whereas in non-thermal food pro cessing complete inactivation might not always occur in a linear fashion65,66. The extent of microbial inactiva tion depends on multiple factors ...
To evaluate the extent of structural conservation for the three species, we compared the exchange behavior of monomeric Pab1 in each species, for which we were able to obtain excellent peptide coverage (FigureS5). We measured proportional deuterium uptake (%D) after 100 seconds of deuterium. ...