Total cell lysates were prepared using SDS-lysis buffer (50 mM Tris–HCl, pH 6.8, 10% glycerol, 1% SDS). Protein content was determined (Micro BCA assay kit, Pierce), and equal amounts of reduced protein (20–70
Cells need to coordinate gene expression with their metabolic states to maintain cell homeostasis and growth. How cells transduce nutrient availability to appropriate gene expression remains poorly understood. Here we show that glycolysis regulates histo
What if ph is equal to pka ?What if ph is smaller than pka?What if ph is bigger than pka? It tells us a bit about degree of dissociation. Somehow I have a feeling you are trying to compare apples and oranges and you don't understand how pH and pKa are related (hint: they are ...
and the pH increases. The pH at which the —CO2H group is one-half neutralized is equal to pK1. After one equivalent of base has been added, the zwitterion is the major form in solution, and the pH at this point is pHi. Addition of a second equivalent of base starts to convert the...
pH = pKa+ log10([A-]/[AH]) Where the square brackets are used to indicate the concentrations of the acid and its conjugate base. The equation may be rewritten as: Ka/[H+] = [A-]/[AH] This shows that pKa and pH are equal when half of the acid has dissociated. The buffering ...
user Flash • Boot from system memory bootloader • Boot from any address in embedded SRAM • Boot from Root Security service (RSS) The BOOT0 value may come from the PH3-BOOT0 pin or from an option bit depending on the value of a user option bit to free the GPIO pad if needed...
What does vary with concentration, in appropriate circumstances, is theapparentpKa estimated by assuming that the pH at the midpoint of the titration is equal to pKa. This comes from the Henderson-Hasselbalch equation: pH = pKa + log([A-]/[HA]) ...
HEK293T cell pellets were lysed in NP40 lysis buffer (1% NP40, 20 mM Tris-HCL pH 7.5, 150 mM NaCl, 1 mM EGTA, 5 mM NaF) with complete protease inhibitor cocktail (Roche) andN-ethylmaleimide (NEM, Sigma). For immunoprecipitation (IP), cleared lysates were added to either Fl...
The characteristics of binary photoelectrodes were studied experimentally to understand the influence of film thickness, cationic solution pH, and post-thermal treatment temperature. Remarkably, the SILAR technique's usage of preheating for CdS deposition, a less frequently employed method, led to ...
Obesity, particularly middle-age obesity, has recently been linked to the induction of DNA damage in adipose tissue due to obesity-induced accumulation of reactive oxygen species and inflammation6. DNA damage triggers the cellular self-rescue processes, including homologous recombination, nonhomologous ...