Transposon-mediated DNA delivery, although first limited to a small number of invertebrate species, has become progressively more applicable to vertebrates, including mammalian cells. This progress has opened the door to the development of a new generation of vectors for human gene therapy and ...
The piggyBac transposon system has proven effective in genomic engineering of mammalian cells for preclinical applications, including gene discovery, simultaneous multiplexed genome modification, animal transgenesis, gene transfer in vivo achieving long-term gene expression in animals, and the genetic ...
enlarged its application areas in transgenic aspects on Pisces,parasite and mammalian.As the cognition of its function and disposition profounds,the piggyBac transposon will be applied in more areas such as gene function and therapy,transposon mutagenesis of cell line,engineered protein expression and ...
PiggyBac Transposon System (ver. 051011-001)PiggyBac™ Transposon Vector System Cat. # PBxxx-1 User Manual Store kit at -20 °C on receipt A limited-use label license covers this product. By use of this product, you accept the terms and conditions outlined in the Licensing and ...
) FACS analysis for mKO2 gene corrected 293-TLR (G) and hiPSC-TLR (H) cells edited with PB-PE g+4 R20P8_dP transposon at different days of puromycin selection. (I) Cell count per well of PB-PE g+4 R20P13_dP-edited 293-TLR cells, after transposon excision by hyPBase or hyPBase_...
Among those transposons, the moth-derived transposon piggybac appears to be the most promising genetic tool due to its higher efficient transposition and higher cargo capacity. Therefore, it can be used for transgenesis, discovery of cancer gene and tumor suppressor gene, and gene therapy. The ...
The transposon, as part of a recombinant protein expression vector, contains the gene(s) of interest (GOI) you want to insert into your host cell, flanked by transposase-recognising inverted terminal repeat (ITR) sequences. Once the vector is transfected into the host cell line along with ...
Engineering of the PB transposase enzyme (PBase) and its cognate transposon DNA elements resulted in a substantial increase in transposition activities. Consequently, this has greatly enhanced the versatility of the PB toolbox. It is now widely used for stable gene delivery into a broad variety of...
piggyBacTransposon=TransposaseSystemtoGenerateCD19-SpecificTCellsfortheTreatmentofB-LineageMalignanciesPallaviV.RajaManuri,1MatthewH.Wil..
piggyBac 转座系统的功能改进及在哺乳动物中的应用