The PB transposase will remove the transposons from the genome, footprint-free. The PiggyBac Transposon System consists of two parts PiggyBac transposase expression vector (PB210PA-1-SBI or PB220PA-1-SBI) PiggyBac transposon vector of your choice The PiggyBac all-in-one inducible vector (PB...
Recently, piggyBac (PB) has been developed as a transposable and efficient gene transfer tool in mammalian cells. In the present study, we developed three types of PB transposon systems containing a dual plasmid system (DPS), a single plasmid system (SPS), and a DNA-mRNA combined system (...
piggyBac 转座系统的功能改进及在哺乳动物中的应用
改进后的 PB 转座系统在基因组研究、基因治疗、诱导多能干细胞(Induced pluripotent stem cells, iPSCs) 诱导及其分化方面发挥了较大的作用。文章对 PB 转座系统的最新研究进展和应用前景进行了综述。关键词: piggyBac;转 3、基因;靶向插入;转座The improvement and application of piggyBac transposon system in ...
PiggyBac 转座子向量系统用户手册说明书 A (ver. 14 - 09122018)PiggyBac™ Transposon Vector System Cat. # PBxxx-1 User Manual Store kit at -20 °C on receipt A limited-use label license covers this product. By use of this product, you accept the terms and conditions outlined...
(ITRs) located on both ends of the transposon vector and efficiently moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. The powerful activity of the piggyBac transposon system enables genes of interest between the two ITRs in the PB vector to ...
PiggyBac Transposon System (ver. 051011-001)PiggyBac™ Transposon Vector System Cat. # PBxxx-1 User Manual Store kit at -20 °C on receipt A limited-use label license covers this product. By use of this product, you accept the terms and conditions outlined in the Licensing and ...
(PB),atransposon= transposasesystem,hasbeenusedtoefficientlygenerateinducedpluripotentstemscellsfromsomaticcells, withoutgeneticalteration.Inthispaper,weapplyPBtranspositiontoexpressachimericantigenreceptor(CAR) inprimaryhumanTcells.WedemonstratethatTcellselectroporatedtointroducethePBtransposonand transposasestably...
The piggyBac (PB) transposon has been used in a number of biological applications. The insertion of PB transposons into the genome can disrupt genes or regulatory regions, impacting cellular function, so for many experiments it is important that PB transposition is tightly controlled. Here, we sy...
报告主题Memory-rich CAR-T cell engineering by piggyBac transposon system for solid malignancies Visit the following website for registration and further details. https://www.labroots.com/webinar/memory-rich-car-t-cell-engineering-piggybac-transposon-system-solid-malignancies...