Taq DNA Polymerase (Cat. No. EP0401). However, before adding the overhangs it is very important to remove all the Phusion DNA Polymerase by purifying the PCR product carefully, as the proofreading activity in Phusion DNA Polymerase is very strong. Any remaining Phusion DNA Polymerase will ...
Phusion DNA Polymerase combines extreme fidelity with unparalleled speed and robustness Superior yields of long PCR products 浏览此商品的顾客同时也查看了 Phusion™ 高保真 DNA 聚合酶 (2 U/μL) Catalog number: F530S 804.00 / Each添加至购物车 DreamTaq Green PCR 预混液 (2X) Catalog number: K108...
Phusion DNA 聚合酶是克隆的理想选择,可用于长片段或难扩增的扩增子。Phusion 是目前具有最高扩增准确性的热稳定聚合酶之一,错配率为TaqDNA 聚合酶的五十分之一,火球菌(Pyrococcus furiosus)DNA 聚合酶(1)的六分之一。Phusion DNA 聚合酶具有 5´→3´ 聚合酶活性和 3´→5´ 核酸外切酶活性,可生成平...
Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), Phusion is one of the most accurate thermostable...
Phusion 是目前具有最高扩增准确性的热稳定型聚合酶之一,错配率不到TaqDNA 聚合酶的 1/50,是火球菌(Pyrococcus furiosus)DNA 聚合酶(1)的 1/6。Phusion DNA 聚合酶具有 5´→3´ 聚合酶活性和 3´→5´ 核酸外切酶活性,可生成平末端产物。
Protocol Phusion® High-Fidelity PCR Master Mix with HF Buffer 使用指南 Activity of Restriction Enzymes in PCR Buffers Guidelines for PCR Optimization with Thermophilic DNA Polymerases 工具& 资源 选择指南 DNA Polymerase Selection Chart Thermophilic DNA Polymerases ...
- Hot start modification of the polymerase prevents amplification of non-specific products and unwanted degradation of primers prior to first cycle of PCR- T4 DNA Ligase included in the kit; no purification steps before or after ligation- Compatible with all strains of competent E. coli cells ...
Relative fidelity values of different DNA polymerases. Phusion DNA Polymerases have extremely low error rates. The error rate, determined by a modified lacI-based method, is approximately 50-fold lower than that of Taq DNA polymerase and 6-fold lower than that of Pfu DNA polymerase. Fidelity =...
- Hot start modification of the polymerase prevents amplification of non-specific products and unwanted degradation of primers prior to first cycle of PCR - T4 DNA Ligase included in the kit; no purification steps before or after ligation
- High fidelity—provides >100X higher sequence accuracy than Taq polymerase - Convenient setup—enables a universal annealing temperature of 60°C in PCR - Enhanced specificity—hot-start mechanism reduces non-specific amplification and primer degradation - Benchtop stability—automation enabled due to ...