- Decrease enzyme concentration. - Optimize Mg2+ concentration. - Titrate template amount. - Decrease primer concentration. - Design new primers. Please read through the following information regarding high molecular weight smears from a Phusion High-Fidelity DNA Polymerase reaction: ...
Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA ...
Less enzyme - superior yield Phusion DNA Polymerase combines extreme fidelity with unparalleled speed and robustness Extreme fidelity for demanding PCR Superior yields of long PCR products Phusion™ High-Fidelity DNA Polymerase (2 U/μL), 100 units ...
results highlight the robustness and speed of Phusion DNA Polymerase. It was able to amplify 94% of the amplicons with lower enzyme amounts still producing superior yields. The success rate of Pfu DNA polymerase was only 56% and that of Taq DNA polymerase 62% with significantly lower yields....
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP...
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP...
- High fidelity (52X Taq)- Fast PCR due to short extension times (15-30 s/kb)- Robust performance, minimal optimization needed- High yields of PCR products with minimal enzyme amounts 组成成分: ▪ Phusion DNA Polymerase (2 U/µL)▪ 5X Phusion HF Buffer▪ 5X Phusion GC Buffer▪...
Extreme Fidelity – >50X greater than Taq. High Speed – Dramatically reduced extension times (10X faster than Pfu) High Yield – Increased product yield using minimal amount of enzyme Versatile – Can be used for routine PCR as well as long or difficult templates 应用特性 PCR Cloning Long or...
Phusion U Hot Start PCR Master Mix is convenient 2X mix containing Phusion U Hot Start DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added by the user. 优点: - Accuracy—high fidelity DNA polymerase (25x Taq) ...
Polymerase Fidelity: What is it, and what does it mean for your PCR? Using aptamers to control enzyme activities: Hot Start Taq and beyond质控、安全 & 法规 质控分析 每一新批次的 NEB 产品都要经过质控,以满足指定的质量标准,对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品...