Please read through the following information regarding high molecular weight smears from a Phusion High-Fidelity DNA Polymerase reaction: - Decrease enzyme concentration. - Decrease extension time. - Reduce the total number of cycles. - Increase annealing temperature or try 2- step protocol. ...
Polymerase Fidelity: What is it, and what does it mean for your PCR? Using aptamers to control enzyme activities: Hot Start Taq and beyond质控、安全 & 法规 质控分析 每一新批次的 NEB 产品都要经过质控,以满足指定的质量标准,对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品...
Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA ...
Phusion Hot Start II DNA polymerase concentration is optimized to give good results in most reactions. When the PCR reaction is set up according to the instructions, the final concentration of Phusion enzyme is 1 U in 50 µL reaction (0.4 U in 20 µL reaction)....
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP...
results highlight the robustness and speed of Phusion DNA Polymerase. It was able to amplify 94% of the amplicons with lower enzyme amounts still producing superior yields. The success rate of Pfu DNA polymerase was only 56% and that of Taq DNA polymerase 62% with significantly lower yields....
Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than of Taq DNA Polymerase and 6-fold lower than that of ...
Extreme Fidelity – >50X greater than Taq. High Speed – Dramatically reduced extension times (10X faster than Pfu) High Yield – Increased product yield using minimal amount of enzyme Versatile – Can be used for routine PCR as well as long or difficult templates 应用特性 PCR Cloning Long or...
than other DNA polymerase. Highlights • High fidelity (52X Taq) • Fast PCR due to short extension times (15-30 s/kb) • Robust performance, minimal optimization needed • High yields of PCR products with minimal enzyme amounts ...
Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA ...