Phusion Hot Start II DNA polymerase concentration is optimized to give good results in most reactions. When the PCR reaction is set up according to the instructions, the final concentration of Phusion enzyme is 1 U in 50 µL reaction (0.4 U in 20 µL reaction). No separate activation ...
Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA ...
Phusion Plus DNA Polymerase is the latest generation of Phusion DNA polymerase that is widely cited in peer-reviewed publications. As a hot-start, proofreading PCR enzyme, Phusion Plus DNA polymerase enables generation of PCR amplicons with high sequence accuracy, sensitivity,...
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP...
results highlight the robustness and speed of Phusion DNA Polymerase. It was able to amplify 94% of the amplicons with lower enzyme amounts still producing superior yields. The success rate of Pfu DNA polymerase was only 56% and that of Taq DNA polymerase 62% with significantly lower yields....
Extreme Fidelity – >50X greater than Taq. High Speed – Dramatically reduced extension times (10X faster than Pfu) High Yield – Increased product yield using minimal amount of enzyme Versatile – Can be used for routine PCR as well as long or difficult templates 应用特性 PCR Cloning Long or...
a novelPyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than ofTaqDNA Polymerase and 6-fold lower than that...
times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase. Highlights • High fidelity (52X Taq) • Fast PCR due to short extension times (15-30 s/kb) • Robust performance, minimal optimization needed ...
than other DNA polymerase. Highlights • High fidelity (52X Taq) • Fast PCR due to short extension times (15-30 s/kb) • Robust performance, minimal optimization needed • High yields of PCR products with minimal enzyme amounts ...
Phusion Hot Start II DNA polymerase concentration is optimized to give good results in most reactions. When the PCR reaction is set up according to the instructions, the final concentration of Phusion enzyme is 1 U in 50 µL reaction (0.4 U in 20 µL reaction). 其他推荐产品 Phusion U...