聚合酶链反应(Polymerase chain reaction,PCR)是一种在体外快速扩增特定基因或 DNA 序列的方法。双链 DNA 分子在临近沸点的温度下加热时分离成两条单链 DNA 分子(变性过程),一对引物分别和两条 DNA 分子特异位置结合(退火过程),在适宜温度下,DNA 聚合酶以单链 DNA 为模板,利用反应混合物中的 4 中脱氧核苷酸为...
Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity PROTOCOL FOR PCR USING TAQ DNA POLYMERASE This is a guideline for PCR amplification with taq DNA polymerase from GenScript. This guideline can help you optimize ...
Taq DNA Polymerase具有5’→ 3’聚合酶活性和5’→ 3’外切酶活性,无3’→ 5’外切酶活性。 TaqDNA Polymerase配合经优化的2 × TaqPCR Buffer (Mg2+ plus),具有极高的扩增效率。 应用: ●常规快速PCR扩增,最高可扩增8 kb 产品规格: 产品组分 NPP101-01 NPP101-02 TaqDNA Polymerase (5 U/ul) 100 ...
Protocol The following procedure is suggested as a guideline and starting point when using Platinum Taq DNA Polymerase High Fidelity in any PCR amplification. Reaction size may be altered to suit user preferences. Add the following components to...
P103Taq DNA Polymerase for PAGE诺唯赞生物 网址/www.vazyme.com 咨询热线/400-600-9335 销售/sales@vazyme.com 技术支持/support@vazyme.com 技术服务/service@vazyme.comVazyme Biotech Co., Ltd Web/www.vazyme.com Tel/400-600-9335 Sales/sales@vazyme.com Support/support@vazyme.com Service/service@...
Protocol for PCR with Hot Start Taq DNA Polymerase How to Avoid ContaminationUp, Reaction Set
本酶由克隆有Thermus aquaticus DNA Polymerase基因的大肠杆菌诱导表达、分离纯化而成,其分子量为94 kDa。Taq DNA Polymerase具有5’→ 3’聚合酶活性和5’→ 3’外切酶活性,无3’→ 5’外切酶活性。 TaqDNA Polymerase配合经优化的2 × TaqPCR Buffer (Mg2+ plus),具有极高的扩增效率。
KAPA Taq DNA Polymerase 高品質、高性能的常規 PCR。 KAPA Taq DNA Polymerase is based on the single-subunit, wild type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus.KAPA Taq 和 KAPA Taq HotStart DNA Polymerase 具有 5'-3「 聚合酶和 5」-3「 外切酶活性,但無 3」-5' 外切酶...
阿仪网为您提供Taq DAN polymerase PCR Buffer(10×)的详细介绍,如有意向请联系上海尚宝生物科技有限公司
Standard PCR Protocol Using Green Taq DNA Polymerase ………. 3 VIII IX Troubleshooting ……… Order Information ………. 6 7 I. INTRODUCTION Green Taq DNA Polymerase is designed to increase the stability of the Taq enzyme for more convenient transport and applications. The polymeras...