Reconstituting Arabidopsis CRY2 signaling pathway in mammalian cells reveals regulation of transcription by direct binding of CRY2 to DNA. Cell Rep. 24, 585–593.e584 (2018). Article CAS PubMed Google Scholar Yang, L. et al. Using HEK293T expression system to study photoactive plant ...
Moreover, the features of channel complexes formed by a given TRPC isoform in distinct cell types or phenotypes are likely variable, giving rise to highly tissue and cell-type specific signaling patterns, which are considered as the basis of TRPC pathologies. Efforts have been undertaken to ...
whereεdenotes absolute permittivity which we assume to be constant. Notably, the difference in the shape of the two monomeric OptoDArG photoisomers drives the changes indhc. In contrast, the recently reported Ziapin2 decreasesdhcdue to spontaneous dimer formation and increasesdhcupon blue light-driv...
The effective control of biointerfacial interactions is of outstanding interest in a broad range of biomedical applications, ranging from cell culture tools to biosensors and implantable medical devices. For many of these applications, highly specific interactions between cells and material surfaces are ...
The University of Edinburgh. This protocol describes a microfabrication-compatible method for cell patterning on SiO2. A predefined parylene-C design is photolithographically printed on SiO2 wafers. Following incubation with serum (or other activation s
Fluorescence-activated cell sorting analysis and fluorescence intensity measurements confirmed the dose-dependent uptake of TagBFP:MAPLEXs by HEK293T cells (fig. S5, A and B). To further examine the uptake of TagBFP:MAPLEXs by recipient cells, HEK293T cells were incubated with HEK293T-derived...
and drug release properties in different conditions. The bioimpacts of NPs on breast cancer cells were studied by evaluating the cell uptake, cytotoxicity, and apoptosis/necrosis regulation. Further, the effectiveness of the chemo/PTT therapy combination of the synthesized NIOs/AuNPs-CS/ARI was invest...
Here we demonstrated that human opsin5 (OPN5) photoreceptor mediates light-induced Ca(2+) response in human embryonic kidney (HEK293) and mouse neuroblastoma (Neuro2a) cell lines using a luminescence imaging system with a...会议名称: Bioluminescence and Chemiluminescence - Light Emission: Biology ...
Global mapping of Salmonella enterica–host protein–protein interactions during infection. Cell Host Microbe 29, 1316–1332 e12 (2021). Article CAS PubMed PubMed Central Google Scholar Tanrikulu, I. C., Schmitt, E., Mechulam, Y., Goddard, W. A. & Tirrell, D. A. Discovery of ...
A stable HEK cell line was created with CYP1B1 expression under the control of tetracycline to facilitate regulated and titratable expression (see the Methods section for more details). A cell line was also created for CYP1A1 as a counter screen to evaluate selectivity. CYP1A1 is the closest...