This study examined variations in media composition during recombinant antibody (IgG) production. Transiently transfected Human embryonic kidney (HEK293) cells and stably transfected IgG expressing Chinese hamster ovary (CHO DG44) cells were grown in serum free media. Supernatant from these cell lines ...
A culture media screening was performed to confirm tyrosine auxotrophy in HEK293 cells [24]. Cells were cultivated in tyrosine-free and tyrosine-containing medium. After 48 h, a significant difference in cell viability was observed, and at day 6 cells did not survive when cultured in tyrosi...
The plasmid kuptake, which directly affects transfection efficiency (percentage of cells effectively transfected), can change depending on experimental conditions, including the chemical structure and molecular weight of the transfection agent, media composition, cell density, cell health, cell line, ...
The highest productivity of this cell line in terms of the E2-CD154 protein quantity expressed per cell was obtained when the cells were grown in the Pro293S-CDM media. This productivity was statistically significant respect to the E2-CD154 protein expressed by the cells growing in CPCHO, CD...
Induction of inflammation in vascular endothelial cells by metal oxide nanoparticles: effect of particle composition. Environ Health Perspect. 2007;115(3):403–409. 26. Sun SY. N-acetylcysteine, reactive oxygen species and beyond. Cancer Biol Ther. 2010;9(2):109–110. 27. Li Z, Hulderman ...
The knockdown of ATM, ATR, and PDEδ in HEK293 cells increased HIV-1 VLP titers in the supernatant by 3.4-, 2.1-, and 2.2-fold, respectively. Also, possible metabolic synergies between plasmids were investigated by statistical design of experiments (DoE), enabling us to identify the optimal...
The need for new safe and efficacious therapies has led to an increased focus on biologics produced in mammalian cells. The human cell line HEK293 has bio-synthetic potential for human-like production attributes and is currently used for manufacturing of several therapeutic proteins and viral vectors...
Mammalian cells are becoming the prevailing expression system for the production of recombinant proteins because of their capacity for proper protein folding, assembly, and post-translational modifications. These systems currently allow high volumetric production of monoclonal recombinant antibodies in the rang...
Explain how bacteria are protected from the body's immune system or defensive cells when they are in a biofilm. Explain how the adaptability of microorganisms increases disease transmission? Explain the various forms of enteric pathogenic strains of Escherichia co...
The HepG2 cells were seeded in 6-well plates (3 × 105 cells/well) and allowed to reach about 80 % confluence. Then, the media was removed and replaced with PBS. Subsequently, a tip was used to scratch the cell monolayer, and the PBS was moved gently to remove the detached cells. ...