The phosphorylation of the peptide, Ser Asp Gln Glu Lys Arg Lys Gln-Ile Ser Val Arg Gly Leu, at the seryl residue between isoleucine and valine by phosphorylase kinase is described. No phosphorylation occurs at the seryl residue at the amino terminus. With this peptide as a substrate, EGTA...
but not with an equivalent amount of PP-1, inactivated (i.e., closed) the channel. Subsequent reaction with MgATP plus the cAMP-dependent protein kinase activated (i.e., opened) the chloride channel. This provided an example of a purified channel protein activated and inactivated by phosphory...
FAK is a non-receptor tyrosine kinase that has a role in cell migration and cell death (McLean et al, 2005). However, conditioned medium only activated p70S6k, but not FAK, while the effects were not different between TP-expressing cells and the non-TP-expressing cells. Therefore, this ...
Possibly activation of TFT by thymidine kinase (TK) is very efficient, preventing inactivation by TP. Trifluorothymidine possibly acts by TS inhibition and DNA incorporation. However, orally administered TFT in combination with TPI (TAS-102) seems to prevent systemic degradation (e.g. liver) of ...
(1989) Reaction of fluorescein isothiocyanate with an ATP binding site on the phosphorylase kinase a-subunit. Eur.J.Biochem. 182, 577-584.Zaman N, Varsanyi M, Heilmeyer LMG, Jr, Sotiroudis TG, Johnson CM, Crabb JW (1989) Reaction of fluorescein isothiocyanate with an ATP-binding site ...
Purine base⇌Pi, Phosphorylaseribose-l-Pribonucleoside →kinaseATP ribonucleotide Although purine nucleoside Phosphorylase is known to function in the catabolism of purine nucleosides (see Chapter 10), there is in fact no evidence that it functions as indicated in this scheme. Thus, in cel...
Factors that have been proposed to make the disorder specific for T-cells include high capacity for deoxynucleoside transport, high expression levels of deoxycytidine kinase, and low levels of phosphatases for dGMP. The normal response to antigenic T-cell stimulation requires clonal expansion from a...
FAK is a non-receptor tyrosine kinase that has a role in cell migration and cell death (McLean et al, 2005). However, conditioned medium only activated p70S6k, but not FAK, while the effects were not different between TP-expressing cells and the non-TP-expressing cells. Therefore, this ...
G., Catalysis of the phos- phorylase kinase activation reaction, J. Biol. Chem., 246, 1968, 1971.Walsh DA, Perkins IP, Brostrom CO, Ho ES, Krebs EG: Cataly- sis of the phosphorylase kinase activation reaction. J Bioi Chern 246:1968-1976,1971...
rmGPa and hlGPa were prepared by phosphorylation of rmGPb and hlGPb, respectively, performed using a truncated form of the γ (catalytic) subunit of rabbit skeletal muscle phosphorylase kinase produced as described previously [26]. Kinetic studies were performed at 30 °C in the direction of ...