significant phosphorylase a accumulation in both isolated atria and right ventricular strips of phosphorylase kinase-deficient mice, and the drug-stimulated increases in phosphorylase a activity and the contractile responses of right ventricular strips were similar in Swiss and phosphorylase kinase-deficient ...
In an assay measuring radioactive incorporation from γ-[ 32 P]ATP into phosphorylase b , cardiac muscle extracts from mice with the phosphorylase kinase deficiency mutation showed significant, calcium-dependent phosphorylase kinase activity that was 10 to 15% of that of Swiss mice, the control str...
Phosphorylase kinase activity has an absolute requirement for Ca2+, which binds to the δ-subunit. The amino acid sequence of this subunit is nearly identical to that of calmodulin, with four calcium binding sites, but unlike calmodulin, the δ-subunit is an integral part of the enzyme and d...
Muscle glycogenosis with low phosphorylase kinase activity: Mutations in PHKA1, PHKG1 or six other candidate genes explain only a minority of cases Muscle-specific deficiency of phosphorylase kinase (Phk) causes glycogen storage disease, clinically manifesting in exercise intolerance with early fatigua...
Specificity for T-cells arises from expression of deoxycytidine kinase which phosphorylates dGuo and repression of phosphatases that hydrolyze dGMP. This was recognized as a potential therapy for T-cell cancers and T-cell autoimmune disorders by the pharmaceutical industry. Numerous patents were...
FAK is a non-receptor tyrosine kinase that has a role in cell migration and cell death (McLean et al, 2005). However, conditioned medium only activated p70S6k, but not FAK, while the effects were not different between TP-expressing cells and the non-TP-expressing cells. Therefore, this ...
The relative expression of TP mRNA was given as the ratio of the concentration NT of TP vs NT of b-actin. Thymidine phosphorylase activity The TP activity was determined using an assay previously described (Laurensse et al, 1988). Activity was measured using thymidine as a substrate by ...
Drug induced suppression of phosphorylase kinase activity correlates with resolution of psoriasis as assessed by clinical, histological and immunohistochemical parameters. Br J Dermatol 2000; 143: 937-949.Heng M C,Song M K,Harker J,et al.Drug-inducedsuppression of phosphorylase kinase activity ...
Src activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Phosphorylation of Tyrosine (Tyr)416 in the activation loop of the kinase domain by carboxy-terminal Src kinase (Csk) upregulates enzyme activity. Phosphorylation of Tyr527 in the carboxy-terminal tail renders ...
Glycolytic potential and activity of adenosine monophosphate kinase (AMPK), glycogen phosphorylase (GP) and glycogen debranching enzyme (GDE) in steer carcasses with normal (< 5.8) or high (> 5.9) 24 h pH determined in M. longissimus dorsi[J] . A. Apaoblaza,A. Galaz,P. Strobel,A. ...