The biosensor consists of a fusion between a fluorescent protein and a lipid-binding domain that specifically binds PtdIns4 P , i.e. the pleckstrin homology domain of the human protein phosphatidylinositol-4-phosphate adaptor protein-1 (FAPP1). YFP–PH FAPP1 was expressed in four plant systems...
that autophagy structures form closely to the PM and that the PM-associated PI4Kα1 is required for proper autophagy flux suggesting an important contribution of the PM to plant autophagy. We speculate that PI4P mediates the recruitment of a yet-unidentified PI4P-binding protein(s) that work(...
inphospholipidtransport. The PtdIns4Palso functions as an important precursor to PtdIns(4,5)P2, which also acts as a membrane recognition domain for interaction with binding proteins. Cleavage of PtdIns(4,5)P2produces two important signaling molecules,diacylglycerolandinositol(1,4,5)-trisphosphate...
The small GTP-binding protein Rho regulates a phosphatidylinositol 4-phosphate 5-kinase in mammalian cells. Integrin-mediated adhesion is known to stimulate production of phosphatidylinositol 4,5-bisphosphate (4,5-PIP2) and increase 4,5-PIP2 hydrolysis in respons... LD Chong,A Traynor-Kaplan,GM...
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B. Binding and release of cholesterol in the Osh4 protein of yeast. Proteins 75, 468–477 (2009) . Article CAS Google Scholar Mousley, C. J. et al. A sterol-binding protein integrates endosomal lipid metabolism with TOR signaling and nitrogen sensing. Cell 148, 702–715 (2012) . ...
The crystal structure of a PH–START complex revealed that the START domain binds to the PH domain at the same site for PtdIns(4)P-binding, suggesting that the START domain competes with PtdIns(4)P for association with the PH domain. We further report that mutations disrupting the PH–...
Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities The second messenger phosphatidylinositol 3,4,5-trisphosphate [PtdIns(3,4,5)P(3)] is generated by the action of phosphoinositide 3-kinase (PI 3-kinase), an... S Dowler,CURRIE, Richard ...
Crystal structure of the phosphatidylinositol 3,4-bisphosphate-binding pleckstrin homology (PH) domain of tandem PH-domain-containing protein 1 (TAPP1): mo... Crystal structure of the phosphatidylinositol 3,4bisphosphate-binding pleckstrin homology (PH) domain of tandem PH-domain-containing protein 1...
B, the effects of mutations in the putative ATP binding domain of PIP5Kα. Kinase activities were normalized with the amounts of immunoprecipitated enzymes estimated by Western blotting. PtdIns(4)P kinase activity of the wild-type α isoform was taken as 100%. Data are presented as means ±...