We rationalised that overexpression of PI3P‐binding GFP‐HrsFYVE competes for PI3P on phagosomal membranes and results in dissociation of PI3P effectors from phagosomes. EhVps26 and EhVps35, but not sorting nexins (SNXs), of the retromer complex were detected from phagosomes only without GFP‐...
PtdIns(3)P is localized primarily in the endomembrane systems of eukaryotic cells and is recognized by proteins with one of several recently identified PtdIns(3)P-binding domains. PtdIns(3)P and these associated proteins play roles in cellular functions associated with the endomembrane system, ...
Phosphatidylinositol 3-phosphate-binding protein AtPH1 controls the localization of the metal transporter NRAMP1 in Arabidopsis. 来自 Semantic Scholar 喜欢 0 阅读量: 173 作者: A Agorio,J Giraudat,MW Bianchi,J Marion,C Espagne,L Castaings,F Lelièvre,C Curie,S Thomine,S Merlot 展开 摘要: "...
Recently we provided some clues that may help answer these questions.1 By following the dynamics of several phosphatidylinositol 3-phosphate (PI3P)-binding proteins during amino acid starvation (and autophagy induction) we concluded that at least some autophagosomes are formed in a starvation-induced,...
To determine whether 3-phosphorylated phosphoinositides are dynamically regulated by light, we developed a highly sensitive assay based on phosphoinositide binding domains and ELISA with chemiluminescence detection that is capable of quantifying low-abundance phosphoinositides at the femtomolar level (see Materi...
After antibody binding at 4 °C and 3 h of treatment with protein synthesis inhibitor (cycloheximide), the 22C11 immu- noreactivity was mostly found at the plasma membrane, as shown by the punctate staining at the basal plane of the cells (Supplementary Fig. S4c). After a 15-min chase ...
Phosphatidylinositol(3)-phosphate signaling mediated by specific binding to RING FYVE domains. Mol. Cell 2, 157-162 (1998). Phosphatidylinositol(3)-phosphate signaling mediated by specific binding to RING FYVE domains. Mol. Cell 2, 157-162.Burd, C.G., and S.D. Emr. 1998. ...
2). This terminus is also thought to be involved in membrane binding and nuclear localization. On the other hand, Gβγ activates β2, β3, and, to a lesser extent, β1 through their PH domains, although the concentrations of Gβγ required for maximal enzyme activation are much higher ...
Overall, these data indicate that by binding to PI(3)P, the E1/E2 complex plays an important role in maintaining the steady-state levels of PI(3)P and, thus, sustains some key PI(3)P-dependent processes, e.g., autophagy.Biochemical and Biophysical Research CommunicationsFanghui HuaCaden ...
Fc R-stimulated activation of the NADPH oxidase: phosphoinositide-binding protein p40phox regulates NADPH oxidase activity after enzyme assembly on the ... The phagocyte NADPH oxidase generates superoxide for microbial killing, and includes a membrane-bound flavocytochrome b(558) and cytosolic p67(phox...