A calculator program is described that will compute the concentrations of mono- and dibasic phosphate salts required for preparation of buffers of desired pH and total phosphate concentration at 25掳C. The program also permits compensation for the presence of other neutral electrolytes in solution and...
so instead of calculating the concentrations of all species at equilibrium to find the buffer's concentration, we should instead find the final concentration of the starting materials (citric acid and sodium phosphate) as i did?hope you don't lose your patience, but i think i have got the p...
This PBST recipe calculator enables the accurate preparation of wash buffer for any milliliter volume. Input your desired volume, click the CALCULATE button, and the table will populate with the amounts of each component needed to make this common buffer used for Western blotting and other immunoa...
Compare http://www.chembuddy.com/?left=concentration&right=dilution-mixingThe fastest approach is to use a buffer calculator, like Buffer Maker. But on some basic level these are rather simple calculations.Besides, I think you should have no problems finding a recipe for a buffered saline, thi...
To remove water-soluble proteins, membranes were resuspended by homogenization in a high-salt buffer containing 20 mM HEPES-NaOH (pH 7.5), 500 mM NaCl, 20 mM MgSO4, 10 mg ml À 1 DNase I, 10 mg ml À 1 RNase A, 1 mM TCEP, 1 mM PMSF and Complete Mini EDTA-free protease ...
Function Buffer View Product On Supplier's Website Sign in or register to save this reagent to your favourites Supplier provided information Applications None provided Experiments from the literature Applications FC/FACS Dilutions None available...
SunRed™ Phosphate working solution (2X): On the day of the experiment, either dissolve SunRed™ Phosphate solid in sterile H2O or thaw an aliquot of the SunRed™ Phosphate stock solution at room temperature. Prepare a 2X working solution of 10 to 50 µM in 100 mM Tris buffer or bu...
(Roche)14. For GUS assays, samples were kept immersed in ice-cold 90% acetone with gentle shaking for 1 h followed by three washes with sodium phosphate buffer pH 7 for 1 h. Tissues were incubated in GUS staining solution for 3 h at 37 °C14and images were taken on a Leica...
The sequencing mixture contained 1 ll of BigDye terminator cycle sequencing kit version 1.1 (Applied Biosystems; ABI, Foster City, CA), 1 ll of amplifica- tion primer or nest primer, 2 ll of BigDye Terminator Sequencing Buffer and 1 ll of distilled water. We performed the cycle sequencing ...