A calculator program is described that will compute the concentrations of mono- and dibasic phosphate salts required for preparation of buffers of desired pH and total phosphate concentration at 25掳C. The program also permits compensation for the presence of other neutral electrolytes in solution and...
http://www.chembuddy.com/?left=Buffer-Maker&right=buffer-calculator « Last Edit: October 17, 2019, 05:41:03 PM by Borek » Logged ChemBuddy chemical calculators - stoichiometry, pH, concentration, buffer preparation, titrations.info Print Pages: [1] Go Up « previous next » ...
Solutions0125 New Member Posts: 3 Mole Snacks: +0/-0 How to prepare 10 mM sodium phosphate buffer, pH 7.2? « on: June 22, 2015, 01:48:01 PM » Hello, I am trying to figure out how to make this buffer properly, but I am getting inconsistent results when I Google this solut...
(Roche)14. For GUS assays, samples were kept immersed in ice-cold 90% acetone with gentle shaking for 1 h followed by three washes with sodium phosphate buffer pH 7 for 1 h. Tissues were incubated in GUS staining solution for 3 h at 37 °C14and images were taken on a Leica...
For large-scale purification of PIP synthases, frozen cell pellets were resuspended in lysis buffer containing 20 mM HEPES-NaOH (pH 7.5), 200 mM NaCl, 20 mM MgSO4, 10 mg ml À 1 DNase I, 10 mg ml À 1 RNase A, 1 mM TCEP, 1 mM phenylmethyl sulfonyl fluoride (PMSF) and ...
On the day of the experiment, either dissolve SunRed™ Phosphate solid in sterile H2O or thaw an aliquot of the SunRed™ Phosphate stock solution at room temperature. Prepare a 2X working solution of 10 to 50 µM in 100 mM Tris buffer or buffer of your choice, pH 8 to 9. SunRed...
The sequencing mixture contained 1 ll of BigDye terminator cycle sequencing kit version 1.1 (Applied Biosystems; ABI, Foster City, CA), 1 ll of amplifica- tion primer or nest primer, 2 ll of BigDye Terminator Sequencing Buffer and 1 ll of distilled water. We performed the cycle sequencing ...