so instead of calculating the concentrations of all species at equilibrium to find the buffer's concentration, we should instead find the final concentration of the starting materials (citric acid and sodium phosphate) as i did?hope you don't lose your patience, but i think i have got the p...
A calculator program is described that will compute the concentrations of mono- and dibasic phosphate salts required for preparation of buffers of desired pH and total phosphate concentration at 25掳C. The program also permits compensation for the presence of other neutral electrolytes in solution and...
The dialysis buffer consisted of 20 mM HEPES- NaOH (pH 7.0), 200 mM NaCl and 0.05% (w/v) DDM. The next day, the sample was removed from the dialysis cassette and purified again using washed Ni-NTA to remove TEV protease, cleaved decahistidine tags and any non-cleaved protein. Flow-...
On the day of the experiment, either dissolve SunRed™ Phosphate solid in sterile H2O or thaw an aliquot of the SunRed™ Phosphate stock solution at room temperature. Prepare a 2X working solution of 10 to 50 µM in 100 mM Tris buffer or buffer of your choice, pH 8 to 9. SunRed...
(Roche)14. For GUS assays, samples were kept immersed in ice-cold 90% acetone with gentle shaking for 1 h followed by three washes with sodium phosphate buffer pH 7 for 1 h. Tissues were incubated in GUS staining solution for 3 h at 37 °C14and images were taken on a Leica...
After culturing for 48 h, NIH3T3 cells were washed with Dulbecco's PBS (Invitrogen) and collected in 1· lysis buffer. Luciferase activity in each construct was assayed using the Dual-Luciferase Re- porter Assay System (Promega) with a luminometer (Lumat LB9507, BERTHOLD, Bad Wildbad, Ger-...