Caldwell J (1979) The significance of phase II conjugation reactions in drug disposition and toxicity. Life Sci 24:571-578.Caldwell J (1979) The significance of phase II (conjugation) reactions in drug disposition and toxicity. Life Sci 24:571–578...
Phase 2 enzymes traditionally refer to the enzymes that catalyze the conjugation reactions of various substrates with endogenous ligands, such as glutathione S-transferase (GST), UDP-glucuronosyltransferase (UGT), N-acetyltransferase (NAT), and sulfotransferase (SULT). However, the term has gradually...
The major metabolic pathways are conjugation (phase II) reactions, whereas oxidative metabolism (O-demethylation) is a minor pathway. BHA is metabolized to its main metabolites 4-O-conjugates, O-sulfates, and O-glucuronides. The metabolites are rapidly excreted through urine with no long-term ...
药物体外代谢研究III- PHASE II酶研究
This array represents genes encoding the seven major classes of phase II drug metabolism enzymes catalyzing such reactions as glutathione conjugation, glucuronidation, sulfation, methylation, amino acid conjugation, epoxidation, and esterification. Using real-time PCR, you can easily and reliably analyze ...
(1 mg in 200 µl) yielding a 6.9 mM dye concentration. The conjugation reaction was done by incubating 500 µl of 358 µM α-Syn-A140C with 200 µl AlexaFluor-488 C-5 maleimide (10-fold excess of dye) for 1 h at room temperature. The reaction solution was...
(1 mg in 200 µl) yielding a 6.9 mM dye concentration. The conjugation reaction was done by incubating 500 µl of 358 µM α-Syn-A140C with 200 µl AlexaFluor-488 C-5 maleimide (10-fold excess of dye) for 1 h at room temperature. The reaction solution was...
GSTs are major phase II metabolizing enzymes that predominantly catalyze the conjugation of reduced GSH to a wide range of hydrophobic, endogenous and exogenous, electrophilic molecules. The GSTs are divided into phylogenetically distinct soluble and membrane bound microsomal families that each contain ...
Conjugation reactions were quenched by addition of 10 mM DTT. Removal of free fluorophore and any free dsDNA was achieved by flowing unlabeled, AF488-labeled, or AF594-labeled histone H2B through 2x5mL desalting columns and Source 15Q resin (GE Healthcare) equilibrated in Histone CleanUp Buffer...
[89] but rather by forming an isopeptide bond with the target protein. This bond is created through the coordinated activity of specific enzymes, namely the E1 SUMO activation enzyme, E2 SUMO conjugation enzyme, and E3 SUMO ligase (Fig.2) [113]. Additionally, members of the SENP family ...