AThe schematic diagram of the 8 + 8 type phage display for GM-CSF presentation on M13 phage.BThe display of GM-CSF on the phage was detected by ELISA assays. Use a HA-tag antibody (left) or a GMCSF antibody (right) as the capturing reagent. Reprinted from Ref. [118] Full size...
We have developed a simple phage based capture ELISA system that enables femtomolar or better detection of individual protein variants directly from complex biological samples. The protocol utilizes a capture reagent that selectively recognizes a unique epitope of the protein variant and a phage based ...
2.2.3.5 Direct ELISA of solubly expressed scFv fragments After the specific binding of phage is detected, single clones are picked for analysis of soluble antibody expression using direct monoclonal ELISA (Fig. 8). The soluble expression antibody is achieved immediately after bio-panning, by infecti...
Phage ELISA ER2738 cells electroporated or infected by panning output phage were plated on a 2 × YT agar plates. Ninety six colonies from each panning condition were selected. The clonal cells were cultured and infected with Hyperphage. After overnight cultivation, the culture supernatant was...
ELISA: enzyme-linked immunosorbent assay MRS: Man-Rogosa-Sharpe broth ORF: open reading frame PBS: phosphate buffered saline PEG: polyethylene glycol SOF22: serum opacity factor of Streptococcus pyogenes, M-type 22 ssDNA: single-stranded DNA Tat: twin arginine translocon.References...
Phage-ELISA was performed according the protocol performed elsewhere36. ELISA measurements were determined at 492 nm (Titertek Plus, Flow Laboratories, USA) and the cut-off values were determined by the ROC curve. Reactivity indices (RI) were obtained by dividing the ELISA readings by the cut...
Further, they have been demonstrated to be functional in a variety of standard immunoassays including Western blotting, ELISA, cellular immunofluorescence, immunoprecipitation and related assays. This methodology will accelerate antibody discovery and ultimately bring us closer to realizing the goal of ...
(bottom panel) the WT-EGFR-ECD-Fc or Mut-EGFR-ECD-Fc fusion protein. NIH3T3 cell-based binding assays were performed by flow cytometry, while EGFR-ECD-Fc fusion protein-related binding assays were performed by ELISA. The Kd value was calculated using nonlinear regression analysis of a one-...
TMB-ELISA substrate was added (50 µl per well), the reaction was stopped by adding 2 N H2SO4 (50 µl per well), and the resulting color developed in each well was measured at 450 nm in a Synergy H1 plate reader (Biotek). For St-Glc1 and St-Glc5v2 binding assay ...
Compared to the conventional ELISA, this electrochemical immunosensor provided a wider linear range (4.4 × 10−3–10 ng/mL) and a 2500-folder lower detection limit as 0.15 ng/mL [101]. A similar approach had been employed for detection of atrazine [102]. Differently, a noncompetitive ...