2.2.3.4 Polyclonal phage enzyme-linked immunosorbent assay (ELISA) Specific antibody-displayed phage are detected by polyclonal phage ELISA (Fig. 7). Sign in to download hi-res image Fig. 7. Illustration of polyclonal phage ELISA. The target antigen is coated on the ELISA plate, followed by ...
The invention discloses a phage display epitope based detection method for influenza A virus antigen blocking ELISA (enzyme linked immunosorbent assay). The method comprises steps of firstly coating an ELISA board with influenza A virus or subtype specificity monoclonal antibody so as to form a ...
ELISA: enzyme-linked immunosorbent assay MRS: Man-Rogosa-Sharpe broth ORF: open reading frame PBS: phosphate buffered saline PEG: polyethylene glycol SOF22: serum opacity factor of Streptococcus pyogenes, M-type 22 ssDNA: single-stranded DNA Tat: twin arginine translocon.References...
In order to construct a phage-display library, we collected peripheral blood from eight COVID-19 convalescent patients after they have provided written informed consent; the RBD-specific antibody titers were then determined in each serum sample by enzyme-linked immunosorbent assay (ELISA). As expecte...
Development of an enzyme-linked immunosorbent assay for the detection of the herbicide clomazone. The development of an ELISA for clomazone (Command) is reported. The ELISA system proved to be a fast, sensitive and accurate method of clomazone detection... FK Koppatschek,RA Liebl,AL Kriz,.....
Measurement of IgG & IgM by ELISA Enzyme-linked immunosorbent assay (ELISA) was used to determine whether blood serum contained levels of antibodies to bacteriophage 9563 in experimental rats. The experiment was performed by the following method: blood was collected from rats at sacrifice and centrif...
An enzyme-linked immunosorbent assay (ELISA) showed that these peptides were reactive with the H7 flagellin in a dose-dependent manner. Among them, a D1 phage clone showed the highest binding affinity to the H7 flagellin. We synthesized the D1 peptide (LHIHRPTLSIQG) corresponding to the ...
The clone VHH-28 resulted in the lowest 50% inhibitory concentration of 0.31 ng/mL in the phage enzyme-linked immunosorbent assay (ELISA) and was selected to develop the VHH phage-based real-time immuno-PCR (RT-IPCR). The detection limit of the VHH phage-based RT-IPCR was 3.7 pg/L,...
Binding affinity of the bound phages was compared with each other by enzyme-linked immunosorbent assay (ELISA), fluorescence imaging technique, and bioinformatic computations. Binding specificity and binding selectivity of the best candidate phage-displayed p16 binding peptide were evaluated by peptide ...
The serum levels of p93 in the normal human and the four diseases patients were detected by Enzyme-linked immunosorbent assay (ELISA), they were also compared with each other. 方法以杂交瘤技术制备p93单克隆抗体;应用酶联免疫吸附测定(ELISA)直接法检测正常人与上述4种疾病患者血清中p93的表达状况及血...