檢驗目標:愛滋病毒抗體。 (1) 酵素免疫分析法 ( Enzyme-Linked Immunosorbent Assay,簡稱 ELISA ):又稱酵素免疫分析法。ELISA可設計出各種不同類型的檢測方式,主要以三明治法 ( Sandwich )、間接法 ( Indirect ) 以及競爭法 ( Competitive ) 三種為主。ELISA 利用抗原抗體之間有專一性的特性進行檢測;即利用抗原所...
HIV 抗体检测是临床判断HIV感染的重要指标,虽然检测方法较多,但对于HIV 抗体的初筛,仍面临着种种困难,不同方法的检验结果存在一定的差异[4]。本研究主要分析AIDS 检验中HIV 抗体酶联免疫吸附剂测定法(enzyme-linked immunosorbent assay,ELISA)的筛查价值,现报道如下。 1 资料与方法 1.1 一般资料 选取2018 年12 月...
Human immunodeficiency virus-1NASBA-ELISA (enzyme-linked immunosorbent assay) detection kitThe invention relates to a gene detection kit against human immunodeficiency virus-1 (HIV-1). The gene detection kit comprises a pair of amplification primers, a capture probe, a detection probe, an NASBA (...
Of an additional panel of eight serum samples which had been found positive in the IDEXX indirect ELISA system, only five were found positive in the antibody inhibition ELISA and western blotting assay.TRUNCATED AT 250 WORDS) 展开 关键词: enzyme-linked immunosorbent assay, Feline immunodeficiency ...
This chapter discusses the enzyme-linked immunosorbent assay (ELISA) as an important means to evaluate host responses to a group of mycoplasmas increasingly associated with various sexually transmitted diseases, including acquired immunodeficiency syndrome (AIDS). These tests have demonstrated a surprisingly...
酶联免疫吸附试验(enzyme-linkedimmunosorbentassay,ELISA)、胶体金法为筛查HIV抗体的重要方法,但目前何种方法检测结果更为准确尚无确切结论[3,4]。本研究探讨ELISA测定HIV抗体对AIDS的诊断价值,并与胶体金法诊断HIV抗体结果进行比较,从而实现AIDS早发现、早诊断、早治疗,现报告如下。
利用特殊旳交联剂研制出辣根过氧化物酶---人血清白蛋白及酸性磷酸酯酶---抗体，统称为酶标识物或酶结合物，用于抗原或抗体旳示踪、定位或定量测定 酶联免疫吸附测定(enzyme-linkedimmunosorbentassay简称为Elisa)---是在免疫酶技术(immunoenzymatictechniques)旳基础上发展起来旳一种新型旳免疫测定技术。本法兼有敏捷...
A Peptide Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of Human Immunodeficiency Virus Type-2 (HIV-2) Antibodies: an Evaluation on Polymerase Chain Reaction (PCR) Confirmed Samples [J]. J Clinical Virology, 2001,22( 1 ): 41-46....
Engvall E, Perlmann P. Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G. Immunochemistry. 1971;8(9):871-874. doi:10.1016/0019-2791(71)90454-X Weemen BKV, Schuurs AHWM. Immunoassay using antigen—enzyme conjugates. FEBS Letters. 1971;15(3):232-236. doi:10.10...
本试剂盒用于人类免疫缺陷病毒1型p24抗原(Human Immunodeficiency Virus Type 1p24 Antigen)的定量检测,适用于人血清(或血浆)及细胞培养上清液等标本,仅供研究使用。 【检验原理】 本产品采用固相酶联免疫吸附分析法(Enzyme-linked Immunosorbent Assay,ELISA)检测人血清(或血浆)中的游离HIV-1 p24抗原和细胞培养上清液等...