Using Purified Human ALP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ALP and ALP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, ...
SensoLyte® pNPP Alkaline Phosphatase Assay Kit to detect alkaline phosphatase activity in biological samples, alkaline phosphatase-based ELISA.
Alkaline Phosphatase, Calf Intestinal (CIAP) Part Numbers: M1821, M2825 Share CIAP catalyzes the Hydrolysis of 5´ Phosphate Groups from DNA, RNA, and r- and dNTPs Available at high concentration; Cat.# M2825 contains 1,000 units of CIAP at 20u/μl Qualified for blue/white cloning; ...
Number of assays: Sufficient material is supplied for 1,000 assays based on the protocol below. Introduction Description of the System The Chemiluminescent Alkaline Phosphatase ELISA detection system incorporates CSPD® or CDP-Star® 1,2-dioxetane substrates for alkaline phosphatase with Sapphire-II...
Alkaline phosphatase (AP)-conjugated secondary antibodies are commonly used in enzyme driven applications, such as western blotting, ELISA, immuno-histochemistry, and others. AP-conjugated secondary antibodies use alkaline phosphatase substrates mainly for chemiluminescent or colorimetric detection. Naviga...
Then, cells were stained with the Alkaline Phosphatase Color Development Kit (Beyotime, China) according to the manufacturer’s protocol. The stained cells were observed by the scanner (ESPON, Japan). Osteogenic differentiation assays MC3T3-E1 cells, mouse pre-osteoblasts or hMSCs were plated in ...
Background: Alkaline Phosphatase/ALPL Several distinct genes encode alkaline phosphatases (APs) in mice with different tissue-specific expression patterns. The Alpl gene, also known as Akp2, encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP) (1). The Alpl ...
Alkaline Phosphatase Rabbit Monoclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。 注意事项: 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意...
A heat labile alkaline phosphatase enzyme and methods of using the same and kits including the same are disclosed. Specifically, a nucleotide sequence of, peptide sequence of, metho
15.A recombinant heat labile alkaline phosphatase comprising:(a) all or a significant part of an amino acid sequence as shown in SEQ ID No. 2; or(b) all or a significant part of an amino acid sequence which has at least 60% sequence identity with SEQ ID No. 2. ...