Simplified scheme of the protonation and deprotonation of the TiO2 surface.Adapted from Beranek, 2011. The hydroxyl groups may have acid (pKa = 2.9) or basic character (pKa = 12.7), if they are bidentate or monodentate, respectively (Herrmann and Boehm, 1969). For these reasons the TiO2 ...
particularly histidine and aspartate, have emerged as molecular pH sensors that translate pH changes into conformational changes of proteins and thus affect their catalytic activity, substrate binding, stability, interaction, aggregation, and localization [151]. The protonation of ...
doi:doi:10.1093/nar/19.13.3744AccountingAuthor information: (1)Department of Biochemistry, University College and Middlesex School of Medicine, London, UK.World Bank,International financial reporting standards :DobrevPlamenDonniniSerenaGroenhofGerritGrubmüllerHelmut...
The OA protonation state was demonstrated to be the main driving force behind the pH-triggered nanostructural transitions in OA/GMO/LL-37 nanocarriers. Hence, the ability to tune the pKaapp of OA provides a strategy to tailor phase transitions and, potentially, activity to specific pH values. ...
Almost all known pH-responsive antibodies sense pH due to histidine residues within their variable regions. pKa value of the histidine side chain is about 6; thus, at pH below 6.0, the histidine side chain is mostly protonated, whereas, at physiologic pH 7.4, it is deprotonated. It was show...
activity in the pH range of pH 6.0–7.0. This might be attributed to the protonation state of the T1 copper-coordinating histidine imidazole rings, which were previously reported to be protonated at pH 5.033and exhibit a pKa of ~634, respectively. The role of the protonated T1-coordinating ...
(H2) is the only residue with a pKain this range of pH, we explored its protonation state. As shown by the corresponding attenuated total reflectance Fourier-transformed infrared spectroscopy (ATR-FTIR) spectra (Fig. 1j), H2is protonated as a positively charged imidazolium group in the low-...
pKa values were calculated by Protofit 2.1rev1 free software licensed under the Gnu General Public License (GPL)33. Preparation of yeast cells modified by calcium ions at different pH and yeast cells at different pH. oS9.0f c0sea0rt reupvrimastie,ad1e5Cs maam(iOnp)lH.eTs)2hw.eA...
The protonation state of each residue was entered manually, and these were determined at each pH using the PDB2PQR server, which performed the pKa calculations by PropKa [71]. This gave the following total charges: +7 (pH 7.0), +17 (pH 4.5) and + 31 (pH 3.5). The Fab A33 ...
pKaSubstrateKinetic behaviorThe pH has a great physiological effect on the catalysis of tyrosinase because protons can act as competitive-type inhibitors. In this work, a reaction mechanism is proposed whereby only the protonation of the free formsEm(met-tyrosinase) andEox(oxy-tyrosinase) has a ...