pKa values may vary considerably depending on the temperature, ionic strength, viscosity, protein binding and other factors. Unless otherwise noted, values listed have been determined from pH-dependent fluorescence measurements at 22°C. Spectra are in aqueous buffers adjusted to >1...
pKa values may vary considerably depending on the temperature, ionic strength, viscosity, protein binding and other factors. Unless otherwise noted, values listed have been determined from pH-dependent fluorescence measurements at 22°C. Spectra are in aqueous buffers adjusted to...
Introduction of electron-withdrawing groups into fluorescein dyes lowers the pKaof the phenolic group to 5 or below, as exemplified by our Oregon Green dyes and their insensitivity to pH changes in the near-neutral pH range.However, these fluorinated fluorescein dyes are s...
6 or β-1,3 linkages of glucans12. Moreover, such a construction of the yeast cell wall results in there being many functional groups on its surface such as phosphate, carboxyl and amino groups. Under appropriate conditions of pH (above pKavalue), these...
If the pH of the feed is above the pKa of the grafted nanobrushes, the carboxylic groups will be deprotonated and swell. Dead end filtration experiments confirm that polyacrylic acid nanobrushes may be grafted from the surface of high flux nanofiltration membranes without significantly impacting the...
The hydroxyl groups may have acid (pKa = 2.9) or basic character (pKa = 12.7), if they are bidentate or monodentate, respectively (Herrmann and Boehm, 1969). For these reasons the TiO2 surface can be protonated or deprotonated depending on the pH value of the aqueous suspension (Fig. 1.12...
The nanoprobe ONM-100, which emits fluorescence at pH values below 6.9 while its fluorescence is quenched at pH values above 6.9, has been successfully applied to identify breast, esophageal, and colorectal tumors, and squamous cell carcinomas in the head and neck region, prior to surgical ...
Almost all known pH-responsive antibodies sense pH due to histidine residues within their variable regions. pKa value of the histidine side chain is about 6; thus, at pH below 6.0, the histidine side chain is mostly protonated, whereas, at physiologic pH 7.4, it is deprotonated. It was show...
pKa values may vary considerably depending on the temperature, ionic strength, viscosity, protein binding and other factors. Unless otherwise noted, values listed have been determined from pH-dependent fluorescence measurements at 22°C. Spectra are in aqueous buffers adjusted to >1 ...
above pKa, the PVP-NH2copolymer starts to aggregate as a result of deprotonation of its pyridinium groups. The data are consistent with a reported PVP pKavalue of approximately 3. The data indicate that the presence of cationic groups such as NH2groups with a molar ratio 4 does significantly ...