HPTS can be introduced into cells by microinjection, electroporation or liposome-mediated delivery or through ATP-gated ion channels. HPTS has also been loaded into cells using a patch pipette. HPTS has been re
What formula do you use to determine if an acid is protonated or deprotonated when given the pH and {eq}pK_a {/eq}? Acid-Base Reactions: Acids and bases mainly react to form neutral solutions, a reaction termed neutralization reaction. protonation of ...
Absorption and fluorescence spectra of the protonated and deprotonated forms of ACMA (A1324) are quite similar. This observation, and the listed pKa value, are reported in published references. Accumulation of the probe in the presence of transmembrane pH gradients results in...
Absorption and fluorescence spectra of the protonated and deprotonated forms of ACMA (A1324) are quite similar. This observation, and the listed pKa value, are reported in published references. Accumulation of the probe in the presence of transmembrane pH gradients results in flu...
The hydroxyl groups may have acid (pKa = 2.9) or basic character (pKa = 12.7), if they are bidentate or monodentate, respectively (Herrmann and Boehm, 1969). For these reasons the TiO2 surface can be protonated or deprotonated depending on the pH value of the aqueous suspension (Fig. 1.12...
The protonated form of the fluorescent probe has a maximum emission at 540 nm, and the maximum of the deprotonated form is at 630 nm. Using two detectors, FC can continuously record the fluorescence intensity at both wavelengths. In fact, the ratio of both fluorescence intensities is a very ...
For RasGRP1, a Ras-specific GEF, His212 has been identified as a pH sensor that activates RasGRP1 when the inside of the cell becomes less acidic. When the charge on His212 changes from positive (protonated) to neutral (deprotonated), the RasGRP1 protein opens up to bind to DAG (diacyl...
Hydrofluoric acid has a pKa of 3.10. When the acid is dissolved in a solution with a pH of 4.62, will the acid be primarily found in its protonated or deprotonated state? Step 1:Read the question carefully and determine what is being asked. ...
In saline solutions buffered with 5 mM ATP, titration experiments revealed a pKa of ~6.9 at 37掳C. Thus, ATP exists in its protonated ATP3 and deprotonated ATP4 in ASL that can range from pH 6 to pH 7.5. To determine the effect of pH on ATP-stimulated Cl secretion through CaCCs, ...
Absorption and fluorescence spectra of the protonated and deprotonated forms of ACMA (A1324) are quite similar. This observation, and the listed pKa value, are reported in published references. Accumulation of the probe in the presence of transmembrane pH gradients results in fluor...