Water is more ionized at body temperature than at room temperature, so neutral pH at body temperature is 6.8; this is the average intracellular pH. Normal extracellular pH (7.4) is slightly alkaline. pKa is the
Uniquely, those dispersions formed gels at pH values less than the microgel's pKa. This behavior was linked to an outer-shell electrostatic repulsive interaction. The data point to a phenomenon, whereby the m-PEGD shells appear to explode at pH values above 7.0. The control microgel prepd.,...
The fluorescent pH indicators used to detect acidic organelles and to follow trafficking through acidic organelles must have a lower pKa than those described in Probes Useful at Near-Neutral pH—Section 20.2. Also, unlike most pH indicators for cytosolic measurements, pH indica...
HPTS has been reported to be less phototoxic than BCECF. (Top) Data Table For a detailed explanation of column headings, seeDefinitions of Data Table Contents Acidic Solution Neutral/Basic Solution Cat. No. MWStorageSolubleAbsECEmSolventAbsECEmSolventpKaNotes ...
The solution is acidic if its pH is less than 7. If the pH is higher, the solution is basic (also referred to as alkaline). Solutions with a pH that is equal to 7 are neutral. Apart from the mathematical way of determining pH, you can also use pH indicators. The most universally ...
In addition, expression of high levels of pHluorin protein could influence a particular luminal pH by buffering, especially if the expected pH of the organelle is near the pKa of the fluorophore, due to the low number free protons compared to the number of pHluorin molecules. 1.3 pH ...
A pH of 7 is neutral, a pH less than 7 is acidic, and a pH more than 7 is basic. How do you calculate the pH of a buffer solution? To calculate the pH of a buffer solution, the Henderson-Hasselbalch equation is used, pH = pka + log(acid/base). If working with a weak acid...
Its pKa ~4.47 and unique pH-dependent fluorescence is an ideal combination for measuring pH in acidic organelles. At near neutral conditions RatioWorks™ PDMPO emits an intense blue fluorescence at ~450 nm when illuminated at its excitation maximum (~333 nm). In more acidic environments, ...
For these types of applications, fluorescein conjugates are less than optimal because they have little sensitivity in the pH range below pH 5.5. The response range can be extended using conjugates of our Oregon Green 488 dye, which has a much lower pKa than fluorescein (4.7 compared ...
The hydroxyl groups may have acid (pKa = 2.9) or basic character (pKa = 12.7), if they are bidentate or monodentate, respectively (Herrmann and Boehm, 1969). For these reasons the TiO2 surface can be protonated or deprotonated depending on the pH value of the aqueous suspension (Fig. 1.12...