In addition to mediating or limiting the absorption, distribution, excretion, and toxicity of many drugs, P-gp is the potential locus of a number of pharmacokinetic drug-drug interactions when two drugs, one a substrate and the other a substrate or inhibitor of the transporter, are co-...
After TMB substrate solution is added, only those wells that contain Permeability Glycoprotein (Pgp), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is...
13-33(January)P-gptissuedistribution.EffluxactivityassociatedwithP-gpwouldreduceintestinaldrugabsorptionwhileenhancingdrugeliminationthroughtheliverandkidney.Atbarriersitessuchastheblood-brainbarrier,testes,orplacenta,P-gpwouldlimittissueexposuretopotentiallytoxicP-gpsubstratecompounds.pgpP糖蛋白药物相互作用专家讲座...
Schisandrol A from Schisandra chinensis Reverses P-Glycoprotein-Mediated Multidrug Resistance by Affecting Pgp-Substrate Complexes. Schisandrol A from Schisandra chinensis reverses P-glycoprotein-mediated multidrug resistance by affecting Pgp-substrate complexes [J]. Planta Med, 2007, 73... WF Fong - ...
These results showed that morphine was a P-GP substrate, and digoxin related transporters such as oatp2 were involved in its transport. Morphine was not substrate for glucose or probenecid-sensitive transporters. 展开 关键词: Morphine transport P-glycoprotein Probenecid-sensitive transport Multidrug ...
8. 底物溶液(TMB Substrate):1×10ml/瓶。 9. 浓洗涤液(Wash Buffer):1×20ml/瓶,使用时每瓶用蒸馏水稀释25倍。 10. 终止液(Stop Solution):1×10ml/瓶(2N H2SO4)。 样品收集、处理及保存方法: 1)血清---操作过程中避免任何细胞刺激。使用不含热原和内毒素的试管。收集血液后,1000×g离心10分钟将血清...
Keratinases reported from bacteria, actinomycetes and fungi had got their pH optima in neutral to alkaline range.25 In the present study, PM2 a classical PGPR, bears keratinolytic activity which optimal conditions were determine as a function of pH, temperature, time and substrate specificity (Fig...
Substrate specificity of deubiquitinating enzymes: Ubiquitin C-terminal hydrolases single dagger. Presents a study conducted on the processing of several ubiquitin derivatives by two human ubiquitin C-terminal hydrolases (UCH) isozymes, in relation to e... Larsen,Christopher,N.,... - 《Biochemistry》...
• Ver: Verapamil is a Pgp substrate that stimulates Pgp ATPase activity and serves here as a positive control for drug stimulation of Pgp ATPase activity. • TC1–TC16: Experimental test compounds. Provide a measure of total ATPase activity in the presence of test compounds. Note: TC1–...
20 μl of specimen was incubated with 2 mmol/l Z-Gly- Pro-pNa for 24 h iline (pNa) from at 37 °C and 5% the substrate is dCeOte2c, taenddscpleecatvraogpehooftopmareat-rnicitarlolyana-t 410 nm and compared to a generated standard curve for PE activity. These methods have ...