Detection Reagent B 1×120µL Assay Diluent B 1×12mL TMB Substrate 1×9mL Stop Solution 1×6mL Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1 Assay procedure summary 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well....
Includes: • 1 vial ATP Detection Substrate (lyophilized) • 10ml ATP Detection Buffer • 10ml Pgp-Glo™ Assay Buffer • 1ml 50mM MgATP • 100μl 10mM Verapamil • 250μl 10mM Na3VO4 (sodium orthovanadate) Storage Conditions: Store Recombinant Human Pgp Membranes at –70°C. ...
8. 底物溶液(TMB Substrate):1×10ml/瓶。 9. 浓洗涤液(Wash Buffer):1×20ml/瓶,使用时每瓶用蒸馏水稀释25倍。 10. 终止液(Stop Solution):1×10ml/瓶(2N H2SO4)。 样品收集、处理及保存方法: 1)血清---操作过程中避免任何细胞刺激。使用不含热原和内毒素的试管。收集血液后,1000×g离心10分钟将血清...
Protein determination, keratinase and protease assay The protein content in culture filtrate and crude enzyme (supernatant) was determined by the Method of Lowry et al.34 Keratinase activity was determined by modified method of Letourneau et al.35 using Keratin as substrate. The keratin was ...
The experimental setup lasted 27 days, with observations carried out in the growth chamber at 24 °C, controlled humidity, and a 15 h photoperiod. The seeds were planted in unsterilized substrate to simulate more realistic natural environmental conditions. The substrate consisted of a soil-sand mi...
Furthermore, nilotinib directly and potently interacts with ABCB1 and ABCG2 at its drug-binding site as shown by photo affinity labelling assay, where nilotinib competitively displaces [125I]-IAAP from its known substrate binding sites of these transporters. This direct interaction of nilotinib was...
Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate Other Materials Required Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1...
Furthermore, nilotinib directly and potently interacts with ABCB1 and ABCG2 at its drug-binding site as shown by photo affinity labelling assay, where nilotinib competitively displaces [125I]-IAAP from its known substrate binding sites of these transporters. This direct interaction of nilotinib was...