Keratinases reported from bacteria, actinomycetes and fungi had got their pH optima in neutral to alkaline range.25 In the present study, PM2 a classical PGPR, bears keratinolytic activity which optimal conditions were determine as a function of pH, temperature, time and substrate specificity (Fig...
While PGP9.5 has been demonstrated to have ubiquitin (ub) carboxy-terminal ethyl esterase activity, there has not been clear identification of its substrate specificity. The main aim was, therefore, to study its carboxyl-terminal hydrolase activity using synthetic ub conjugated peptides. PGP9.S is...
The experimental setup lasted 27 days, with observations carried out in the growth chamber at 24 °C, controlled humidity, and a 15 h photoperiod. The seeds were planted in unsterilized substrate to simulate more realistic natural environmental conditions. The substrate consisted of a soil-sand mi...
20 μl of specimen was incubated with 2 mmol/l Z-Gly- Pro-pNa for 24 h iline (pNa) from at 37 °C and 5% the substrate is dCeOte2c, taenddscpleecatvraogpehooftopmareat-rnicitarlolyana-t 410 nm and compared to a generated standard curve for PE activity. These methods have ...
用99Tcm-MIBI术中γ探测法探讨活体肺部恶性肿瘤组织对99Tcm-MIBI的摄取和肿瘤细胞P-糖蛋白(Pgp)表达间的关系。结果表明,T/NT≤2的肺部恶性肿瘤组织Pgp含量明显高于T/NT>2的肺部恶性肿瘤组织;Pgp含量多寡与病理类型无关;肺部恶性肿瘤组织对99Tcm-MIBI的摄取与Pgp表达呈明显负相关,即肿瘤细胞高度表达Pgp时,99Tcm-...
High MRP-1 cell membrane protein expression might be useful to identify those patients that are most likely to benefit from more aggressive treatment, combination treatment with standard chemotherapeutics and MRP-1 inhibitors, or treatment with a non-MRP-1 substrate (Roundhill and Burchill, 2012)....
l. larvae on MYPGP substrate with a 97% efficiency and could distinguish between MYPGP substrates with 1-2 days of culture, and substrates with older cultures. The efficacy of copies of the prototypes M1 and M2 was shown to differ slightly. The weighted method with Canberra metrics (Canberra...