同时,TOF患者心肌线粒体DNA相对拷贝数在统计学上显著增高(图1)。TFAM是一种核编码因子,可转运到线粒体,与线粒体DNA的D环结合,激活其复制和转录。TFAM是由PGC-1α通过与NRFs共活化而调控的。据报道,PGC-1α表达的增加也增加了线粒...
TFAM (D5C8) Rabbit mAb #8076 100 µl NRF1 (D9K6P) Rabbit mAb #46743 100 µl DRP1 (D6C7) Rabbit mAb #8570 100 µl Product Information Product Usage Information ApplicationDilution Western Blotting 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 ...
老百姓看到的是“体育锻炼”,但是北美洲的富豪们看到的是“我要雇人代替我锻炼,我只要每隔三天输液一次他们的血浆”。玩笑了,这种调控机制的中心环节就是“PGC-1a”,以及其下游调控因子NRF1/2、TFAM等。 PGC-1a:过氧化物酶体增殖物激活受体γ-共激活因子-1a。怎么理解呢?有一种叫“受体γ”,它能够让过氧化物...
骨骼肌细胞通过暴露在咖啡因或者Ca2+离子载体A-23187中导致的胞浆Ca2+升高会引起一系列线粒体生物发生的标志,以及线粒体转录因子A(Tfam),过氧化物激活受体γ(PPARγ)和共激活因子1α(PGC-1α)蛋白的增加。在能量紧张这种条件下会导致NRF-1的DNA结合力的增加,δ-氨基乙酰丙酸合成酶mRNA表达的增加,PGC-1α和细...
In VHL wild-type cells, PGC-1a suppression leads to decreased expression of the mitochondrial tran- scription factor Tfam and impaired mitochondrial respiration. Conversely, PGC-1a expression in VHL- deficient cells restores mitochondrial function and induces oxidative stress. ccRCC cells expressing PGC...
The latter studies also provided evidence that the transcription factor NRF1/2 mediates the effects of PGC-1a on the expression of nuclear genes encoding mitochondrial proteins including the mitochondrial transcription factor (TFAM), which is essential for the initiation of mtDNA transcription and ...
结果DOX以浓度-依赖性的方式降低AC16细胞存活率,增加线粒体内ROS水平,降低线粒体膜电位,减少线粒体DNA拷贝数;DOX降低线粒体新生相关蛋白PGC-1a,NRF1,TFAM,VDAC和COXⅣ蛋白表达水平,抑制线粒体新生;DOX降低p62蛋白表达水平,增加Beclin1蛋白表示水平,同时增加LC3Ⅱ蛋白表达水平,诱导AC16细胞发生自噬;此外,DOX促进...
P组即离体MCs过表达目的基因PGC-1α后再经12mg/ml D-半乳糖作用120hr诱导缺失拟老化模型组.通过实时定量PCR检测各组mtDNA4834缺失突变率.通过western blot检测四组中PGC-1α,NRF-1,Tfam,NF-κB,FOXO-1和SIRT-1的蛋白表达水平的变化.通过β-半乳糖苷酶染色检测各实验组细胞的衰老情况.通过TUNEL检测各实验组...
(a) Protein expression of PGC-1a and TFAM were evaluated by immunoblot analysis in Nara-H cells that were co-transfected with PGC-1a plasmid (or control plasmid) and TFAM siRNA (or control siRNA). (b) Relative mtDNA numbers in plasmid/siRNA transfected Nara-H cells were assessed by qRT-...
We generated HON cultures from a patient harbouring the homozygous c1309T>G, W437G PINK1 mutation, and an age and sex-matched control. Real time polymerase chain reaction was performed for relative expression of PGC-1a, Tfam and SOD1, and Western blot was performed for PGC-1a and b-actin...