同时,TOF患者心肌线粒体DNA相对拷贝数在统计学上显著增高(图1)。TFAM是一种核编码因子,可转运到线粒体,与线粒体DNA的D环结合,激活其复制和转录。TFAM是由PGC-1α通过与NRFs共活化而调控的。据报道,PGC-1α表达的增加也增加了线粒...
结论:葱白提取物能够改善 NAFLD 模型大鼠肝脂肪变性,其机制可能与 增加 PGC-1α 的转录活性,促进 NRF-1、TFAM 的表达进而增加线粒体合成有关。 关键词:葱白提取物;非酒精性脂肪肝;肝线粒体;PGC-1α;NRF-1、TFAM To Observe Effects of Fistular Onion Stalk Extract on The Expression of PGC-1Α and ...
hUC-MSC preserves erectile function by restoring mitochondrial mass of penile smooth muscle cells in a rat model of cavernous nerve injury via SIRT1/PGC-1a/TFAM signaling Mengbo Yang, Xinda Chen, Ming Zhang, Xiaolin Zhang, Dongdong Xiao, Huiming Xu & Mujun Lu Biological Research vol...
TFAM (D5C8) Rabbit mAb #8076 100 µl NRF1 (D9K6P) Rabbit mAb #46743 100 µl DRP1 (D6C7) Rabbit mAb #8570 100 µl Product Information Product Usage Information ApplicationDilution Western Blotting 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 ...
PGC一1a的生理功能 1. 1 PGC-1 与骨骼肌线粒体生物合成 骨骼肌组织具有高度的适应性,耐力运动与训 练可以提高骨骼肌细胞的呼吸能力以及抗疲劳能 作者简介:乌日古木拉(1986~),蒙古族,硕士研究 生。研究方向:分子数量遗传学与马的遗传育种 ◇2011年第12期 ...
1a 是 哺乳 动物 中线 粒体 生物 发生的主 导调 控 因子 的数据 还在 不断 积累。肌管中 PGC一1 的异位 表达 强 烈诱 导 了下 游转录 因子 如 NRFs 和 Tfam 的表 达1. ,然 而 ,与 NR Fs 或 T ram 不同 ,PGC一1a 的水 平与 心肌 和骨 骼肌氧化能力相关 ,表明 PGC一1a 在 ...
(常压空气暴露)、HBO组(0.20 MPa)暴露l、5、14、28 d组(HBO l、5、14、28 d);暴露结束后立即处死并取其肝组织,提取肝组织总DNA及总蛋白后,采用qPCR实验检测Mt-DNA的拷贝数,Western blotting实验检测超氧化物歧化酶2(SOD2)、线粒体转录因子A(TFAM)、解偶联蛋白2(UCP2)和AMPK/PGC.1a信号通路蛋白的表达...
qPCR analyses showed a significant decrease in mRNA levels of ERRα, ERRγ, NRF1 and TFAM in ARPE19-PGC1A KO compared to control cells, whereas ERRβ mRNA levels were significantly increased (Fig. 6A). We inhibited PGC1A with siRNA and obtained similar results in ARPE19 cells (S-Fig. ...
1A). Accordingly, mRNA expression levels of mitofusin (Mfn), a mitochondrial fusion-related gene, and mitochondrial transcription factor A (Tfam), which represents mitochondrial mass, were significantly decreased, whereas that of dynamin-related protein 1 (Drp1), a mitochondrial fission-related gene,...
Of note, the expression of genes associated with mitochondrial biogenesis (Nrf-1 and Tfam) was significantly up-regulated by NHP (Fig. 4d). These results suggested that the elevation fatty acid oxidation by NHP might be due to increased mitochondrial biogenesis in liver. NHP improves mitochondrial...