Peptide-N-Glycosidase F (high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins) (PNGase F)| 基本信息 更多信息 中文名称: 中文同义词: 英文名称: Peptide-N-Glycosidase F (high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins) (PNGase F) 英文同义...
Immobilized Peptide N〨lycosidase F (PNGase F) onto magnetic nanoparticles: a biotechnological tool for protein deglycosilation under native conditionsdeglycosylationglycanglycomic analysisimmobilizationnanoparticlespeptide N‐glycosidase FThe elucidation of glycans biological function is essential to understand...
PNGaseF peptide-N-glycosidase F RT reverse transcription LEL large extracellular loop TRX thioredoxin MBP maltose-binding protein ELISA enzyme-linked immunosorbent assay HBS HEPES-buffered saline Hepatitis C virus (HCV)1infection frequently causes chronic hepatitis (1, 2) and frequently progresses to li...
resistant31,49,50, we used Peptide:N-glycosidase F (PNGaseF), an enzyme that digests all N-linked glycans, to validate the US9 SP status of the chimeras (Fig. S3a, annotated beneath the panel). We assessed the cellular localization of US8 and the chimeras by an endoH assay (Fig....
Specificity of enzymatic in vitro glycosylation by PNGase F: a comparison of enzymatic and non-enzymatic glycosylation Enzymatic in vitro glycosylation is possible using a reverse reaction of peptide- N-glycosidase F (PNGase F), and non-enzymatic in vitro glycosylation occu... HY Jeong,YL Ji,HP...
1 The abbreviations used are: RP, reversed-phase; ANN, artificial neural network; PNGase F, peptide N-glycosidase F; DHB, 2,5-dihydroxybenzoic acid; ID, inner diameter; ABRF, Association of Biomolecular Resource Facilities; PRG, Proteomics Research Group. ...
The hGLP-1R is a target for the treatment of type 2 diabetes and belongs to the class B family of GPCRs. Like other class B GPCRs, the GLP-1R contains an N-terminal signal peptide (SP) and undergoes N-linked glycosylation, which are important for its tra
Using peptide: N-Glycosidase F (PNGase-F) to generate glycopeptide with uniform glycosylation patternsdoi:US20050118672 A1This invention provides methods for modifying glycosylation patterns of glycopeptides, including recombinantly produced glycopeptides. Also provided are glycopeptide compositions in which ...
corticotrophin-releasing factor; Glu-C, endoproteinase Glu-C; GLP1, glucagon-like peptide-1; Lys-C, endoproteinase Lys-C; PNGase F, N-glycosidase F; PTH, parathyroid hormone; STI, soybean trypsin inhibitor; TM, transmembrane; Bis-Tris, 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane...
(C) Z-VAD-fmk inhibits YPng1 and not other glycosidases. Endo A, Endo H, PNGase F, and YPng1 (at 3 U/reaction) were incubated for 90 min at room temperature with denatured RNaseB in the presence or absence of 100 μM Z-VAD-fmk (IC50 for 3 U of YPng1). +CHO and −CHO...