Takara 4450 Glycopeptidase F (Peptide:N-glycosidase F) 25 mU ￥1,261 收藏产品 加入购物车 □ 酶浓度 500 mU/ml □ 制品形态: 溶液 组成:20 mM磷酸钠缓冲液pH7.2,50 mM EDTA,0.05% NaN3溶液。 □ 添附Buffer A. Denature buffer : 1% SDS/1 M Tris-HCl (pH 8.6) 500 μl B. Native...
英文名称:Glycopeptidase F(Peptide:N-glycosidase F) 货号:4450 有效物质含量:生物级别 % 包装规格:25 mU □ 酶浓度500 mU/ml □ 制品形态: 溶液 组成:20 mM磷酸钠缓冲液pH7.2,50 mM EDTA,0.05% NaN3溶液。 □ 添附Buffer A. Denature buffer :1% SDS/1 M Tris-HCl (pH 8.6)500 μl ...
Peptide-N-Glycosidase F (high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins) (PNGase F)| 基本信息 更多信息 中文名称: 中文同义词: 英文名称: Peptide-N-Glycosidase F (high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins) (PNGase F) 英文同义...
Enzymatic in vitro glycosylation using peptide- N -glycosidase F[J] . Ji Youn Lee,Tai Hyun Park.Enzyme and Microbial Technology . 2002 (6)Lee and Park, " Enzymatic in vitro glycosylation using peptide-N-glycosidase F, " Enzyme and Microbial Technology 30(6):716-720, (2002)....
Enzymatic in vitro glycosylation is possible using a reverse reaction of peptide- N-glycosidase F (PNGase F), and non-enzymatic in vitro glycosylation occu... HY Jeong,YL Ji,HP Tai - 《Enzyme & Microbial Technology》 被引量: 22发表: 2004年 Agaricus bisporus lectin binds mainly O-glycans ...
11365169001N-Glycosidase F, 100 u100 U (0.1 ml) 11684302001ABTS Solution3 x 100 ml 11719408001Protein A-Agarose2 ml 10884308001CPRG250 mg 11814524001rGFP50 µg 10109878001TRYPSIN INHIBITOR, 1 g1 g (non-sterile) 11080725001Neuraminidase (Sialidase) f. V1 U ...
Artefacts formed by addition of urea to N-linked glycans released with peptide-N-glycosidase F for analysis by mass spectrometry. Husby G et al (2004) Artefacts formed by addition of urea to N -linked glycans released with peptide- N -glycosidase F for analysis by mass spectromet... LA Om...
Human and bovine LFs (10 μg each; Sigma) were denatured with 0.5% SDS, 1% 2-mercaptoethanol for 10 min at 100 °C and were then treated with 2,000 units of peptide-N-glycosidase F (PNGaseF; New England Biolabs) in 50 mm sodium phosphate, pH 7.5, 1% Nonidet P-40 for 4 h at...
et al. DNA N-glycosidases: Properties of uracil-DNA glycosidase from Escherichia coli. J. Biol. Chem. 252(10), 3286–3294 (1977). Article CAS PubMed Google Scholar Chang, A. et al. A novel phage display vector for selection of target-specific peptides. Protein Eng Des Sel 33, gz...
Treatment of the photolabeled ORL1 receptor withN-glycosidase F results in a discrete radioactive macromolecular species of ∼40 kDa (Fig. 3), in good agreement with the value calculated from the amino acid sequence of the ORL1 receptor. The receptor thus appears to be heavily glycosylated ...