Takara 4450 Glycopeptidase F (Peptide:N-glycosidase F) 25 mU ￥1,261 收藏产品 加入购物车 □ 酶浓度 500 mU/ml □ 制品形态: 溶液 组成:20 mM磷酸钠缓冲液pH7.2,50 mM EDTA,0.05% NaN3溶液。 □ 添附Buffer A. Denature buffer : 1% SDS/1 M Tris-HCl (pH 8.6) 500 μl B. Native...
Heat denaturation in sodium dodecyl sulfate rendered these glycoproteins susceptible to peptide:N-glycosidase, but perturbation with chaotropic salts provided a more gentle approach, which was as effective as detergent-unfolding and more compatible with the stability of the enzyme. Once exposed by the ...
英文名称:Glycopeptidase F(Peptide:N-glycosidase F) 货号:4450 有效物质含量:生物级别 % 包装规格:25 mU □ 酶浓度500 mU/ml □ 制品形态: 溶液 组成:20 mM磷酸钠缓冲液pH7.2,50 mM EDTA,0.05% NaN3溶液。 □ 添附Buffer A. Denature buffer :1% SDS/1 M Tris-HCl (pH 8.6)500 μl ...
Peptide-N-Glycosidase F (high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins) (PNGase F)| 基本信息 更多信息 中文名称: 中文同义词: 英文名称: Peptide-N-Glycosidase F (high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins) (PNGase F) 英文同义...
The in vitro glycosylation of pentapeptide (Arg-Lys-Asp-Val-Tyr; RKDVY) and RNase A was carried out using PNGase F (peptide-N-glycosidase F), and the resul... SJ Park,JY Lee,TH Park - 《Journal of Microbiology & Biotechnology》 被引量: 15发表: 2003年 Identification and characterization...
11365169001N-Glycosidase F, 100 u100 U (0.1 ml) 11684302001ABTS Solution3 x 100 ml 11719408001Protein A-Agarose2 ml 10884308001CPRG250 mg 11814524001rGFP50 µg 10109878001TRYPSIN INHIBITOR, 1 g1 g (non-sterile) 11080725001Neuraminidase (Sialidase) f. V1 U ...
Peptide N-Glycosidase F Peptide nucleic acid Peptide nucleic acid Peptide nucleic acid Peptide nucleic acid Peptide nucleic acid Peptide Nucleic Acid Oligomer Peptide nucleic acids Peptide of 18 Residues Peptide PEC-60 homolog peptide phage display peptide phage display peptide receptor radionuclide therapy...
Human and bovine LFs (10 μg each; Sigma) were denatured with 0.5% SDS, 1% 2-mercaptoethanol for 10 min at 100 °C and were then treated with 2,000 units of peptide-N-glycosidase F (PNGaseF; New England Biolabs) in 50 mm sodium phosphate, pH 7.5, 1% Nonidet P-40 for 4 h at...
(called glycans or glycosides) to specific amino acid residues by covalent bonds. Glycosylation of protein occurs in the endoplasmic reticulum and Golgi apparatus and is catalyzed by glycosyltransferases and glycosidases. Glycosylation is one of the most common and complex post-translational modifications...
et al. DNA N-glycosidases: Properties of uracil-DNA glycosidase from Escherichia coli. J. Biol. Chem. 252(10), 3286–3294 (1977). Article CAS PubMed Google Scholar Chang, A. et al. A novel phage display vector for selection of target-specific peptides. Protein Eng Des Sel 33, gz...