pEGFP-C2 Vector哺乳细胞表达载体 产品信息: 货号 产品名称 规格 价格(元) MF3405-2UG pEGFP-C2 Vector哺乳细胞表达载体 2μg 800 【注意事项】: (1) 我司提供的所有质粒(载体)仅保证质粒(载体)的关键部位测序正确,不保证实验效果。 (2) 商业化的质粒(载体)即使是开发公司也未做过完整测序。如果测...
not unique. The Xba I site cannot be used for fusions since it contains an in-frame stop codon. The Xba I and Bcl I sites (*)are methylated in the DNA provided by BD Biosciences Clontech. If you wish to digest the vector with these enzymes, you will need to transform the vector ...
pEGFP-C2VectorInformationPT3051-5 GenBankAccession#:U57606Catalog#6083-1 pEGFP-C2 4.7kb MluI(1646) DraIII(1876) ApaLI (4364) StuI (2581) AseI (8) NheI(592) Eco47III(597) AgeI(601) EcoO109I (3858) SnaBI (341) BsrGI(1323)
into the MCS will be expressed as fusions to the C terminus of EGFP if they are in the same reading frame as EGFP and there are no intervening stop codons. SV40 polyadenylation signals downstream of the EGFP gene direct proper processing of the 3' end of the EGFP mRNA. The vector ...
into the MCS will be expressed as fusions to the C terminus of EGFP if they are in the same reading frame as EGFP and there are no intervening stop codons. SV40 polyadenylation signals downstream of the EGFP gene direct proper processing of the 3' end of the EGFP mRNA. The vector ...
pressingvectorwithEcoRIandBamHIsites.TheserecombinantpEGFP—C2一hG3BP—Domain(1—5、plasmidsweretransfect— edintoHeLacellsandtheexpressionofgreenfluorescentfusionproteinswasexaminedbyfluorescencemicroscopeand Westernblottingassay.Results:Thedomain(1-5)fragmentsofG3BPweresequencedcorrectlyanddetectedintheproducts ...
转染后的PRDX3基因mRNA及蛋白质水平均明显上调.结论 成功构建了具有报告基因-增强绿色荧光蛋白(EGFP)基因的真核表达载体pEGFP-C2-PRDX3,为进一步研究PRDX3基因在人髓母细胞瘤中的生物学功能奠定了基础.%Purpose To construct a human cukaryotie expression vector pEGFP-G2-PRDX3 and to inves...
关键词: 日本血吸虫;脱尾童虫;表膜结合肽;转染效率;抗虫效果 关键词(英文): hybrid protein;animal;article;gene vector;genetic transfection;genetics;larva;metabolism;nucleotide sequence;plasmid;Schistosoma japonicum;Animals;Base Sequence;Genetic Vectors;Larva;Plasmids;Recombinant Fusion Proteins;Schistosoma japoni...
3 - 87 Construction of NPM-pEGFP Fusion Protein Expression Vector
5) pEGFP-C2 vector pEGFP-C2载体 1. METHODS:The mouse GATA4 gene fragment was amplified by PCR from eukaryotic expression plasmid pEFSA-GATA4 and subcloned into pEGFP-C2 vector. 结果:酶切、测序结果表明将基因片段GATA4正确插入pEGFP-C2载体,并可在P19细胞中检测到GATA4基因和GATA4-EGFP融合蛋白...