pEGFP-C3 Vector哺乳细胞表达载体 产品信息: 货号 产品名称 规格 价格(元) MF3406-2UG pEGFP-C3 Vector哺乳细胞表达载体 2μg 800 【注意事项】: (1) 我司提供的所有质粒(载体)仅保证质粒(载体)的关键部位测序正确,不保证实验效果。 (2) 商业化的质粒(载体)即使是开发公司也未做过完整测序。如果测...
into a dam– host and make fresh DNA.pEGFP-C3 Vector Information PT3052-5 GenBank Accession #: U57607Catalog #6082-1 (1638)(2573)Ase I (597)I(601)Eco (3850)G I(1323)Specific EGFP Monoclonal Antibody for Westerns, IP and IC V i sit our website for more details!click here…
unique. The Bcl I site cannot be used for fusions since it contains an in-frame stop codon. The Xba I and Bcl I sites (*) are methylated in the DNA provided by BD Biosciences Clontech. If you wish to digest the vector with these enzymes, you will need to transform the vector into...
The vector backbone also contains an SV40 origin for replication in mammalian cells expressing the SV40 T-antigen. Aneomycin resistance cassette (Neor), consisting of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and poly adenylation signals from the Herpes simplex virus ...
3) pEGFP-C2 vector pEGFP-C2载体 1. METHODS:The mouse GATA4 gene fragment was amplified by PCR from eukaryotic expression plasmid pEFSA-GATA4 and subcloned intopEGFP-C2 vector. 结果:酶切、测序结果表明将基因片段GATA4正确插入pEGFP-C2载体,并可在P19细胞中检测到GATA4基因和GATA4-EGFP融合蛋白表达...
eukaryotic expression vector pEGFP-C3真核表达载体pEGFP-C3 3)pEGFP-C2 vectorpEGFP-C2载体 1.METHODS:The mouse GATA4 gene fragment was amplified by PCR from eukaryotic expression plasmid pEFSA-GATA4 and subcloned into pEGFP-C2 vector.结果:酶切、测序结果表明将基因片段GATA4正确插入pEGFP-C2载体,并可...
pEGFP-C3_医药卫生_专业资料。pEGFP-C3 Vector Information GenBank Accession #: U57607 PT3052-5 Catalog #6082-1 Ase I ApaL I (435 pEGFP-C3 Vector Information GenBank Accession #: U57607 PT3052-5 Catalog #6082-1 Ase I ApaL I (4356) pUC ori P CMV IE (8) SnaB I (341) Nhe I (592...
Quantification of Methylation at CMV Promoter of pEGFP-C3 Vector by Gradient Touch-Down PCR Sequencing基因亚硫酸氢钠测序法甲基化梯度降落PCRCMV启动子pEGFP-C3目的改进亚硫酸氢钠测序法,并在CMV基因启动子甲基化检测中进行验证.方法提取PEgfP-C3质粒重组人肝癌细胞株HEPg2 dnA,亚硫酸氢钠化学修饰,针对修饰后...
3) eukaryotic expression vector pEGFP-C3 真核表达载体pEGFP-C3 4) pEGFP-C1 plasmid pEGFP-C1质粒 1. Construction of anti-CD44 antibody targetedpEGFP-C1 plasmidcomplex and its specifical transfection into rat renal epithelial cells; 方法:以免疫组织化学染色观察大鼠肾小管上皮细胞(NRK52E)表面CD44受体的...
the MCS will be expressed as fusions to the C terminus of EGFP if they are in the same reading frame as EGFP and there are no intervening stop codons. SV40 poly adenylation signals downstream of the EGFP gene direct proper processing of the 3' end of the EGFP mRNA. The vector backbone ...