PE_PGRS62基因克隆重组耻垢分枝杆菌为构建表达牛分枝杆菌(M.bovis)PE_PGRS62蛋白的重组耻垢分枝杆菌,本研究以M.bovis基因组DNA为模板PCR扩增PE_PGRS62基因,获得大小约为1 515 bp的目的片段,并克隆到大肠杆菌-分枝杆菌穿梭表达载体pMV261中,将重组pMV-PE_PGRS62穿梭表达载体电转化到耻垢分枝杆菌内,42℃热诱导...
克隆重组耻垢分枝杆菌生物学活性为构建表达牛分枝杆菌(M.bovis)PE_PGRS62蛋白的重组耻垢分枝杆菌,本研究以M.bovis基因组DNA为模板PCR扩增M.bovis PE_PGRS62基因,获到大小约为1515bp的目的片段.将扩增的PE_PGRS62基因克隆到大肠杆菌-分枝杆菌穿梭表达载体pMV261中,经电转化方法,将重组pMV-PE PGRS62穿梭表达载体...
Expression of PE_PGRS62 in Mycobacterium smegmatis , a nonpathogenic species naturally deficient in PE_PGRS genes, resulted in enhanced resistance to various in vitro stresses and cellular survival in macrophage. As a consequence, the cytokine profiles of macrophage were disturbed and cell apoptosis ...
active tuberculosis protein are associated with latent and pe-pgrs62 mycobacterium tuberculosis strong antibody responses to 来自 dx.doi.org 喜欢 0 阅读量: 6 作者:W Kah,EK Shu,GT Soh,Seah DOI: 10.1128/iai.01175-08 年份: 2019 收藏 引用 批量引用 报错 分享 ...
为构建表达牛分枝杆菌(M.bovis)PE_PGRS62蛋白的重组耻垢分枝杆菌,本研究以M.bovis基因组DNA为模板PCR扩增M.bovis PE_PGRS62基因,获到大小约为1515bp的目的片段.将扩增的PE_PGRS62基因克隆到大肠杆菌-分枝杆菌穿梭表达载体pMV261中,经电转化方法,将重组pMV-PE PGRS62穿梭表达载体转化到耻垢分枝杆菌(M.s...
PE_PGRS62基因克隆重组耻垢分枝杆菌为构建表达牛分枝杆菌(M.bovis)PE_PGRS62蛋白的重组耻垢分枝杆菌,本研究以M.bovis基因组DNA为模板PCR扩增PE_PGRS62基因,获得大小约为1 515 bp的目的片段,并克隆到大肠杆菌-分枝杆菌穿梭表达载体pMV261中,将重组pMV-PE_PGRS62穿梭表达载体电转化到耻垢分枝杆菌内,42℃热诱导...
Thi EP, Hong CJ, Sanghera G, Reiner NE (2013) Identification of the Mycobacterium tuberculosis protein PE-PGRS62 as a novel effector that functions to block phagosome maturation and inhibit iNOS expression. Cell Microbiol 15: 795-808.Thi, E. P., Hong, C. J., Sanghera, G. & Reiner, ...