Structural basis and designing of peptide vaccine using PE-PGRS family protein of Mycobacterium ulcerans—An integrated vaccinomics approachBuruli ulcerImmunoinformaticsMulti-epitope vaccineMycobacterium ulceransPE-PGRS proteinBuruli ulcer is an emerging tissue-necrosis infectious disease, caused by the ...
In this study, we generated and characterized anMtbH37Rv mutant (MtbΔ33) in which the structural gene of PE_PGRS33, a prototypical member of the protein family, was inactivated. We showed that this mutant entered macrophages with an efficiency up to ten times lower than parental or ...
The highly homologous PE_PGRS (Proline-glutamic acid_polymorphic GC-rich repetitive sequence) genes are members of the PE multigene family which is found only in mycobacteria. PE genes are particularly abundant within the genomes of pathogenic mycobacter
bovis BCG have a large number of PE_PGRS proteins, and there may be a lot of functional overlap and redundancy amongst members of this family, role of an individual protein in these systems may not be clearly illustrated either by overexpression of the test protein or by using the gene ...
The PE domain of the PE_PGRS protein has a proline– glutamic acid sequence near the amino terminus and the PGRS domain of the protein varies in size and contains many repeats of alanine and glycine. 1 There is evidence that at least some members of this gene family are expressed on the...
Research progress on the PE_PGRS33protein of Mycobacterium tuberculosis JU Xiao-hong,WANG Yue-hiia,FANG Fang (J iLin Medical University,J itin132013,China)Abstract：The PE_PGRS33protein is a member of the proline—glutamic_polymorphic GC—rich repeat sequence(PE_ PG R S)sub family,which is...
In this review, we summarize the actual knowledge on this elusive protein family in terms of evolution, structure, and function, focusing on the role of PE_PGRS in TB pathogenesis. We provide an original hypothesis on the role of the PE domain and propose a structural model for the ...
Mycobacterium tuberculosis H 37 Rv (Mtb) proteome consists of unique PE_PGRS family proteins, which present a significant role in bacterial pathogenesis over the past years. Earlier evidence suggests that some PE_PGRS proteins display fibronectin-binding activity. In this manuscript, computational ...
The PE_PGRS protein family, characterized by a special N‐terminal PE (Pro (P)〨lu (E) motif) domain and a C‐terminal PGRS (Polymorphic GC‐rich Repetitive Sequences) domain, is restricted mainly to pathogenic mycobacteria. Here we summarize current literature on the PE_PGRS as vital ...
tuberculosis predicted allelic diversity in the PE-PGRS family, and this was confirmed by immunoblot studies as size variants were detected in clinical strains. Subcellular fractionation studies and immunoelectron microscopy localized many PE-PGRS proteins in the cell wall and cell membrane of M. ...